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New

Phosphatidylglycerol Assay Kit (Fluorometric)

The first kit in the market that detects Phosphatidylglycerol levels in biological samples
Catalog #: K488

In stock

$455.00

Product Details

Cat # +Size K488-100
Size 100 assays
Kit Summary Phosphatidylglycerol Assay Kit (Fluorometric): Fluorometric Assay for measuring PG levels in biological samples such as tissue lysate. Detection limit: 0.02 nmol per sample. 100 assays.
Detection Method Fluorometric (535/587 nm)
Species Reactivity All
Applications • Measurement of PG content of various tissue/cell extracts
• Determination of PG concentration in biological fluids
Features & Benefits • Can be completed in less than one hour
• Specific
• Validated with biological samples
• Simple
• Easy protocols
Kit Components • PG Converter Mix
• PG Enzyme Mix
• PG Assay Buffer
• PG Standard (0.5 µmol)
• PG Probe (in DMSO)
Storage Conditions - 20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Phospholipids are physiologically critical amphiphilic molecules that are involved in regulation of numerous cellular processes. Phosphatidylglycerol (PG) is a glycerophospholipid consisting of a glycerol backbone with two acyl chains and a phosphatidyl group that carries another glycerol moiety. Lung surfactant is an important lipid-protein mixture that aids in expansion and prevents total collapse of the lung during respiration. PG is a prominent phospholipid in lung surfactant, and its presence can be monitored as a measure of lung development in the maturing fetus. In addition, most bacteria utilize PG in their membrane, and plants utilize this component as the major phospholipid in the photosynthetic membranes of leaf tissue. In HPLC analysis of phospholipids, phosphatidic acid can interfere with and complicate determination of PG content. BioVision’s Phosphatidylglycerol Assay Kit utilizes specific enzymes to generate an intermediate that reacts with a probe, yielding a signal that can be quantified fluorometrically, and is proportional to the amount of PG present in the sample, unaffected by the presence of phosphatidic acid, cardiolipin, or other lipids. When used as described, the assay is highly selective against other phospholipids (see figure below) and capable of detecting as little as 20 pmoles of phosphatidylglycerol.


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