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New

Phosphatidylethanolamine Assay Kit (Fluorometric)

The first kit in the market to measure Phosphatidylethanolamine in samples
Catalog #: K499
$535.00

Product Details

Cat # +Size K499-100
Size 100 assays
Kit Summary • Detection method- Fluorescence (Ex/Em 535/587 nm)
• Sample type- cell and/or tissue lipid extracts
• Species reactivity- Mammalian
• Applications- This assay provides a convenient and non-ELISA alternative to detect Phosphatidylethanolamine amount in biological samples.
Detection Method Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Mammalian
Applications This assay provides a convenient and non-ELISA alternative to detect Phosphatidylethanolamine amount in biological samples.
Features & Benefits Simple, fast, does not require lenghty incubation times
Kit Components • PE Assay Buffer
• PE Converter
• PE Developer
• PE Enzyme Mix
• Probe
• PE Standard (1 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Phosphatidylethanolamine (PE), also known as cephalin is the second most abundant phospholipid in animal and plant tissues, and is present on the cytoplasmic side of the plasma membrane. Phosphatidylethanolamine is a neutral phospholipid consisting of a phosphatidyl group ester linked to an ethanolamine molecule. Its functions include membrane fission/fusion, maintenance of membrane curvature and stabilization of membrane proteins, since it can form hydrogen bonds with proteins through an ionizable amine group. It acts as a chaperone during assembly of membrane proteins and aids in their translocation from the cytoplasm to the membrane. It is also involved in secretion of very low density lipoproteins in the liver. BioVision’s Phosphatidylethanolamine assay kit is a microplate based enzymatic assay for the quantitation of PE in cells and tissues. PE Converter hydrolyses PE to an intermediate, which converts a colorless probe to a fluorescent product via enzymatic reaction (Ex/Em: 535/587). The intermediate formed through PE converter hydrolysis is specific to phosphatidylethanolamine. Thus no other phospholipids (i.e. phosphatidylcholine, phosphatidylinositol or phosphatidic acid) will be detected, making the kit highly specific. This assay kit can detect as low as 0.2 nmol per well.


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Can we use mouse serum or plasma samples with this kit? Have you tested it?
The assay does not work with mouse serum or plasma samples due to poor recovery rate and sample interference. Therefore, we do not recommend K499-100 assay for serum or plasma samples.