Product Details
Cat # +Size | K410-500 |
---|---|
Size | 500 assays |
Detection Method | Absorbance (650 nm) |
Species Reactivity | Mammalian |
Applications | Phosphate concentrations between 1 µM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined. |
Features & Benefits | • Simple procedure; takes ~ 3 hours • Fast and convenient • The assay is sensitive and stable • The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections. |
Kit Components | • Phosphate Reagent • Phosphate Standard (10 mM) |
Storage Conditions | RT |
Shipping Conditions | RT |
USAGE | For Research Use Only! Not For Use in Humans. |
Details
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2. Presence of IgG and IgM paraproteins can produce false increase in Phosphate concentration by producing precipitates in the reaction. Dilution of the sample can reduce the interference. Deproteination of the samples works best (Clin Chem. 1995 Apr;41(4):60914.).
3. It's advisable to avoid TritonX during sample preparation
4. Some reports suggests Trichloroacetic acid and Tungtic acid can interfere with the assay
5. EDTA, oxalate and citrate should be avoided
6. Significant interference can be caused by hemolysate, bilirubin and intralipids.