Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

Phenylalanine Fluorometric Assay Kit

Catalog #: K572

In stock

$365.00

Product Details

Cat # +Size K572-100
Size 100 assays
Detection Method Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Mammalian
Applications The assay is linear in the range from 0.1 to 1.0 nmol (2-20 µM) of Phenylalanine.
Features & Benefits • Simple procedure; takes less than ~30 minutes
• Fast and convenient
Kit Components • Phenylalanine Assay Buffer
• Tyrosinase
• Enzyme Mix
• Developer
• Phenyalanine Standard (10.0 µmol)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin. Errors in PHE metabolism lead to phenylketonuria or PKU which can have dire consequences. BioVision’s Phenylalanine Assay Kit provides a quick, simple, accurate method for quantifying PHE in biological samples. In the assay, PHE is reductively deaminated with the simultaneous formation of NADH which reacts with our fluorescent probe to generate fluorescence at Ex/Em=535/587 nm. The assay is linear in the range from 0.1 to 1.0 nmol (2-20 µM) of Phenylalanine.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.
What can interfere with the assay?
High concentration of NADH, NADPH and Tyrosine can interfere with the assay causing high background.
Do we need to deproteinize our samples?
"Deproteinization helps remove proteins/enzymes which can degrade/use-up phenylalanine. Deproteinization can be done by Biovision kit K808 (acid precipitation method) or by centrifugation through spin filter columns (cat#1997). The 10 KDa mol wt cut off ensures all higher molecular weight proteins/enzymes are removed from the sample and the flow through contains small molecules only. The maximum loading volume for the spin filter columns is 500 ul and the stop volume is 20-25 ul meaning no matter how long you spin, once 20-25 ul is achieved inside the filter insert, there will be no further flow-through. This is the lost volume for the sample. Typically, for the K572 assay, up to 50 ul sample per well can be used. For samples with small volume, the sample might need to be diluted with water for use with the spin filter. The PCA precipitation could be easier and will prevent any dilutions."
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
 BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.