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Phenylalanine Assay Kit (Colorimetric)

Catalog #: K481

In stock

$365.00

Product Details

Cat # +Size K481-100
Size 100 assays
Kit Summary • Detection method- Absorbance (OD: 450 nm)
• Species reactivity- Mammalian
• Applications- The assay is linear in the range from 1.0 to 10 nmol (1-50 µM) of Phenylalanine.
Detection Method Absorbance (450 nm)
Species Reactivity Eukaryotes
Applications Estimation of Phenylalanine in various biological samples
Features & Benefits • Simple procedure; takes less than ~60 minutes
• Fast and convenient
Kit Components • Phenylalanine Assay Buffer
• Tyrosinase
• Phenylalanine Enzyme Mix
• Phenylalanine Developer
• Phenyalanine Standard (1.0 µmol)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

L-Phenylalanine (Phe) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids building the polypeptide backbone. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin. Errors in Phe metabolism lead to phenylketonuria or PKU which can have dire consequences. BioVision’s Phenylalanine Assay Kit (Colorimetric) provides a quick, simple, accurate method for quantifying Phe in biological samples. In the assay, Phe is metabolized with the simultaneous formation of NADH which reacts with a probe to generate absorbance that can be followed colorimetrically at 450 nm. The assay is linear in the range from 0.2 to 10 nmol, allowing accurate determination of concentrations in the range of 1 - 50 µM Phenylalanine.


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