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Phenylalanine Ammonia-Lyase Activity Assay Kit (Fluorometric)

To measure the activity of Phenylalanine Ammonia-Lyase in samples
Catalog #: K2055
$525.00

Product Details

Cat # +Size K2055-100
Size 100 assays
Kit Summary BioVision's Phenylalanine Ammonia-Lyase Activity Assay Kit measures the activity of PAL via a two-step plate-based assay. The products of the PAL enzymatic reaction, generated in the first step, react with the developers resulting in the formation of a fluorescent compound, which is detected at Ex/Em = 410/470 nm. The kit can detect as low as 3 µUnits (3 pmol substrate catalysis per minute) of PAL under assay conditions.
Detection Method Fluorescent (Ex/Em = 410/470 nm)
Species Reactivity Mammalian
Applications Measurement of Phenylalanine ammonia-lyase activity in samples
Features & Benefits • The assay is simple, sensitive, and can detect as low as 3 µU (3 pmol substrate catalysis/minute) of PAL under assay conditions.
• A facile, rapid way to measure Phenylalanine ammonia-lyase activity in samples.
Kit Components • PAL Assay Buffer
• PAL Substrate
• Developer A
• PAL Positive Control
• Ammonium Chloride Standard
• Microplate Sealing Film
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Phenylalanine Ammonia-Lyase (PAL, EC 4.3.1.24) catalyzes the reversible non-oxidative deamination of L-phenylalanine to trans-cinnamic acid and ammonia without any external cofactors. PAL is expressed in plants, yeast and some fungi and is absent in bacteria and animals. Patients suffering from the genetic disorder, phenylketonuria is unable to metabolize phenylalanine that leads to irreversible cognitive impairment. Historically, phenylketonuria has been treated with a phenylalanine-restricted diet taken for the entirety of the patient’s life. Recently, a drug containing PEGylated PAL has been FDA-approved to treat phenylketonuria, limiting the need of a restricted diet. BioVision's Phenylalanine Ammonia-Lyase Activity Assay Kit measures the activity of PAL via a two-step plate-based assay. The products of the PAL enzymatic reaction, generated in the first step, react with the developers resulting in the formation of a fluorescent compound, which is detected at Ex/Em = 410/470 nm. The kit can detect as low as 3 µUnits (3 pmol substrate catalysis per minute) of PAL under assay conditions.


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