Phenylalanine Ammonia-Lyase Activity Assay Kit (Fluorometric) (ab284571)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate (12 x 8 well strips)
- Sample type: Purified protein, Serum
- Sensitivity: 3 µU
Overview
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Product name
Phenylalanine Ammonia-Lyase Activity Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Serum, Purified protein -
Assay type
Enzyme activity -
Sensitivity
>= 3 µU -
Species reactivity
Reacts with: Mammals -
Product overview
Phenylalanine Ammonia-Lyase Activity Assay Kit (Fluorometric) (ab284571) (K2055) measures the activity of PAL via a two-step plate-based assay. The products of the PAL enzymatic reaction, generated in the first step, react with the developers resulting in the formation of a fluorescent compound, which is detected at Ex/Em = 410/470 nm. The kit can detect as low as 3 µU (3 pmol substrate catalysis/minute) of PAL under assay conditions.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K2055 Phenylalanine Ammonia-Lyase Activity Assay Kit (Fluorometric). K2055-100 is the same size as the 100 test size of ab284571.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Ammonium Standard II 1 x 100µl Detection Reagent 1 x 1.2ml Microplate Sealing Film 1 unit PAL Assay Buffer 1 x 40ml PAL Positive Control 1 vial PAL Substrate 1 vial
Images
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Ammonium Chloride Standard Curve
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Pooled normal human male AB serum was spiked with 5.5, 9.3, 12.7 and 20.6 µU of PAL respectively. The corresponding units of PAL recovered were 4.3, 8.8, 13.2 and 19.7 µU (recovery rates ranging from 78.4- 103.7%). The data shown is the average of three replicates where experiments were performed according to the kit protocol.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab284571 has not yet been referenced specifically in any publications.