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PER2 (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of PER2 in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E4991
$895.00

Product Details

Cat # +Size E4991-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit to quantitatively measure PER2 in human serum, plasma, tissue lysate, and other biological fluids
Features & Benefits ● Recovery range: 85 - 105% for normal human serum and plasma samples
● This Sandwich ELISA is highly sensitive and highly specific for the detection of PER2 in human samples. There is no significant cross-reactivity or interference between PER2 and analogues
● Assay Precision: Intra-Assay CV and Inter-Assay CV < 10%
● Detection range: 0.313 – 20 ng/ml
● Sensitivity: 0.188 ng/ml
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate Sealers
● Standard (Lyophilized) (20 ng)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Period circadian protein homolog 2 (PER2) belongs to the family of Period proteins. It is primarily expressed in the central nervous system (SCN) and peripheral nervous system. As a core component of the circadian clock, it regulates daily rhythms of locomotion, metabolic and behavioral activity. PER2 is involved in the transcription-translation feedback loop wherein it forms a heterodimer with CRYs in the cytoplasm and inhibits CLOCK-BMAL1 transcriptional activity. Mutations of PER2 cause a phenotype similar to the Familial advanced sleep phase. PER2 may also be associated with neurodegenerative diseases, depression, drug addiction, and food anticipation, although mechanisms remain unknown. BioVision’s PER2 (Human) ELISA kit quantitatively measures PER2 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of PER2 in the sample or standard.


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