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PER1 (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of PER1 in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E4989
$795.00

Product Details

Cat # +Size E4989-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit to quantitatively measure PER1 in human serum, plasma, tissue lysate, and other biological fluids
Features & Benefits ● This Sandwich ELISA is highly sensitive and highly specific for the detection of PER1 in human samples. There is no significant cross-reactivity or interference between PER1 and analogues
● Detection range: 15.625 – 1000 pg/ml
● Assay Precision: Intra-Assay CV and Inter-Assay CV < 10%
● Sensitivity: 9.375 pg/ml
● Recovery range: 85 - 105% for normal human serum and plasma samples
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate Sealers
● Standard (Lyophilized) (1000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Period circadian protein homolog 1 (PER1) is a master regulator of the circadian clock that belongs to the period family of proteins. It is expressed in almost all the cells of peripheral tissues, the most notable expression being in the suprachiasmatic nucleus (SCN), a primary circadian pacemaker in the brain. PER1 is involved in a transcription-translation feedback loop wherein it is activated by CLOCK-BMAL1 heterodimer inside the nucleus. PER1 then heterodimerizes with CRYs in the cytoplasm and reenters the nucleus to inhibit CLOCK-BMAL1 transcriptional activity. Besides playing a critical role in the entrainment of the circadian clock to the light-dark cycles, PER1 also regulates the cell cycle and response to DNA damage. Aberrant expression or altered rhythms of PER1 were associated with multiple cancers such as prostate, colon, breast, head and neck, and endometrial cancer. BioVision’s PER1 (Human) ELISA kit quantitatively measures PER1 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of PER1 in the sample or standard.


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