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PEG Virus Precipitation Kit

based on 7 citations in multiple journalsPEG Virus Precipitation Kit74.2 4
High Viral Titer, No ultracentrifugation
Catalog #: K904
SKU-Size Size Price Qty
K904-50 50 preparations
$315.00
K904-200 200 preparations
$875.00
More Sizes Get Quote

Product Details

Detection Method N/A
Species Reactivity N/A
Applications The kit can be used for small lab samples or large scale virus preparation with high yield and high viral titer.
Features & Benefits • A quick, easy and inexpensive method is desired to concentrate virus and remove impurities
• Easy, convenient and time-saving method to concentrate virus without ultra-centrifugation
• Virus can be concentrated over 100 folds
• The whole process uses non-toxic reagents
Kit Components • PEG Solution (5X)
• Virus Re-suspension Solution (1X)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Virus was usually produced at a low titer. It often needs to be concentrated for storage or further applications. A quick, easy and inexpensive method is desired to concentrate virus and remove impurities. BioVision’s PEG Virus Precipitation Kit provides an easy, convenient and time-saving method to concentrate virus without ultra-centrifugation. The kit can be used for small lab samples or large scale virus preparation with high yield and high viral titer. The kit can be used to concentrate retroviruses, baculoviruses, lentiviruses, and phages etc. in cell culture medium or environmental samples. Virus can be concentrated over 100 folds. An optimized Virus Re-suspension Solution is provided to maximize viral recovery by 40-100% depending on the virus type and sources. The whole process uses non-toxic reagents. The concentrated virus can be used for infection, viral DNA or RNA purification, etc.


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I am unable to centrifuge at 4°C as I do not have a cooling system. Will centrifuging at RT affect the virus titer?
4°C is meant to keep the activity low until it is needed. Usually viral preparations are done at lower temperatures. RT may affect the viral titer as it may lead to the degradation of the lipid membrane.
Will PEG precipitation affect the activity of the purified virus?
PEG precipitation is a well-known methodology for concentrating low titer viruses and PEG is a non-toxic polymer. This methodology does not affect the infectious capability of the virus.
Could you please tell me the pH of 1X Virus Resuspension Buffer as I would like to make sure that it is safe to use the purified virus for in vivo injections?
The 1X Virus Resuspension Buffer has a pH 7.2.
Chanyang Kim, et al., (2018) IGF-1 protects SH-SY5Y cells against MPP+-induced apoptosis via PI3K/PDK-1/Akt pathway, Endocrine Connections, May 2018, 29459421
Andrews et al., High Preexisting Serological Antibody Levels Correlate with Diversification of the Influenza Vaccine Response. J. Virol., Mar 2015; 89: 3308 - 3317.
Lee et al., First Report of Oryctes rhinoceros nudivirus (Coleoptera: Scarabaeidae) Causing Severe Disease in Allomyrina dichotoma in Korea. J Insect Sci, Mar 2015; 15: 26.
Warnes et al., Inactivation of Murine Norovirus on a Range of Copper Alloy Surfaces Is Accompanied by Loss of Capsid Integrity. Appl. Envir. Microbiol., Feb 2015; 81: 1085 - 1091.
Busskamp et al., Rapid neurogenesis through transcriptional activation in human stem cells. Mol Syst Biol, Nov 2014; 10: 760.
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