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pCREB Transcription Factor Activity Assay Kit (Colorimetric)

A 96-well plate based colorimetric assay to measure the activation of pCREB in nuclear extracts or cell lysates.

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: K2109
$695.00

Product Details

Cat # +Size K2109-100
Size 100 assays
Detection Method Absorbance at 450 nm
Species Reactivity Mouse, Rat, Human
Applications Measure the activation of pCREB (human, mouse and rat) in nuclear extracts or cell lysates.
Features & Benefits The kit offers easy, rapid, sensitive and non-radioactive way to detect the activation of pCREB transcription factor in samples.
Kit Components ● Plate Coated with DNA Probes
● Non-Competitor Oligo
● TMB Substrate
● Stop Solution
● Positive Control
● Plate Sealing Film
● Binding Buffer (5X)
● DTT (100 mM)
● Protease Inhibitor Cocktail
● pCREB Primary Antibody
● Antibody Diluent Buffer
● HRP Conjugate Stock
● Wash Buffer (10X)
● Competitor Oligo
Storage Conditions Multiple Temperatures
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not For Use in Humans.

Details

CREB (cAMP response element-binding protein) is a phosphorylation-dependent cellular transcription factor. It is activated by phosphorylation by various serine-threonine kinases such as PKA (cAMP-dependent protein kinase A), PKC (protein kinase C) and CaMKs (calmodulin kinases) on the serine 133 residue. Once activated, pCREB interacts with the coactivator proteins and binds to the cAMP response elements (CRE) in the promoter region at 5’ upstream region and increases or decreases the transcription of genes including c-fos, BDNF, tyrosine hydroxylase, numerous neuropeptides etc. CREB is involved in regulating a variety of cellular processes, including cell survival, cell proliferation, glucose homeostasis, spermatogenesis, circadian rhythm, synaptic plasticity associated with memory etc. BioVision’s pCREB Transcription Factor Activity Assay is a 96-well plate based colorimetric assay to measure the activation of pCREB in nuclear extracts or cell lysates. The kit offers easy, rapid, sensitive and non-radioactive way to detect the activation of transcription factors in samples. In this assay, double stranded DNA sequence containing the CRE is coated on the 96-well plate. Active pCREB in the cell lysate or the nuclear extract binds to the oligonucleotides on the plate. After the addition of pCREB primary antibody that recognizes the pCREB-CRE comlex, a HRP-conjugated secondary antibody is added followed by the addition of TMB substrate and a color signal is developed, which is measured at 450 nm.


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