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PCR-Listeria monocytogenes Detection Kit

PCR based Listeria monocytogenes Detection Kit
Catalog #: K1449

Product Details

Cat # +Size K1449-96
Size 96 Rxns
Kit Summary PCR-Listeria monocytogenes Detection Kit is based on amplification and detection of specific DNA fragments from Listeria monocytogenes by real-time PCR method.
Detection Method Real-time PCR
Species Reactivity Listeria monocytogenes
Applications Real-time PCR
Features & Benefits • Amplification limit: 10 UG per reaction (100%), 2 UG per reaction (40%)
• Inclusivity: Positive in 48 reference strains of L. monocytogenes (serovars 1 to 7) from different collections.
• Reliable and highly specific Results.
• Detection: probe labelled with fluorescent dyes Listeria monocytogenes: FAM-BHQ1a; IAC: HEXBHQ1.
• Fast: results in less than 24 hours with minimal handling.
• Quantification limit: 20 UG per reaction (100%)
• Quantification Dynamic range: 6 logs
• Exclusivity: 100% Tested with 94 nontarget strains composed of 51 strains of Listeria nomonocytogenes and 43 strains of non-Listeria.
• Easy and Ready-to-use Kit.
Kit Components • PCR Negative Control
• PCR Master Mix
• PCR Positive Control
• DNA Ready Lysis Buffer
Storage Conditions -20°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not For Use in Humans.


BioVision’s PCR-Listeria monocytogenes Detection Kit is an ideal tool for simple, fast and reliable Amplification and Detection of specific DNA fragments from Listeria monocytogenes by the real-time PCR method. The Kit includes all reagents required in a comfortable ready-to-use Multiplex PCR MasterMix. The optimized MasterMix contains a Buffer, dNTPs, Hot-start DNA polymerase, DNA-free water, MgCl2 and an Internal Amplification Control (IAC) whose detection indicates the absence of PCR inhibitors. Primers and Probes for the amplification of IAC as well as for the amplification of the target gene are included in the MasterMix. The Probe for the detection of target gene is labelled with the FAM, whereas the probe for the detection of IAC is labelled with the HEX fluorochrome. In addition, the kit includes both Positive Control and Negative Control. The Positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNAready Lysis Buffer to extract the DNA from the sample prior to PCR detection.

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Is your kit compatible with the ABI 7500 PCR system?
Yes, it is compatible with ABI 7500 PCR system. Additionally, it is also compatible with Agilent Mx3005P, Applied Biosystems 7300 and other cyclers.