Product Details
Cat # +Size | K1451-96 |
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Size | 96 Rxns |
Detection Method | Real-time PCR method |
Species Reactivity | Legionella spp and/or Legionella pneumophila |
Applications | An ideal tool for an fast and reliable Amplification and Detection of specific DNA fragments from Legionella spp and/or Legionella pneumophila by the real-time PCR method (Multiplex PCR). |
Features & Benefits | • Fast: results in less than 24 hours with minimal handling. • Inclusivity: Primers and probes have been tested satisfactorily in 14 strains of L. pneumophila and 30 species of the genus Legionella. • Amplification limit: Legionella spp: 5 UG per reaction. L. pneumophila: 100 UG per reaction. • Detection: probe labelled with fluorescent dyes– Legionella: FAM-BHQ1a; L. pneumophila: HEXBHQ1; IAC:CY5-BHQ2. • Quantification limit: Legionella spp: 50 UG/L. L. pneumophila: 1000 UG/L. • Exclusivity: No cross-reactions with other frequent bacterial agents have been observed. • Simultaneously detect and differentiate Legionella spp. and L. pneumophila in the same tube • Reliable and highly specific Results. • Thermal cycler: Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers • Quantification Dynamic range: 5 logs • Easy and Ready-to-use Kit. |
Kit Components | • Multiplex PCR Master Mix
• PCR Positive Control • PCR Negative Control • DNA Lysis Buffer |
Storage Conditions | -20°C |
Shipping Conditions | Dry Ice |
USAGE | For Research Use Only! Not For Use in Humans. |
Details
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