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Product Details
| Cat # +Size | K1450-96 |
|---|---|
| Size | 96 Rxns |
| Detection Method | PCR |
| Species Reactivity | Legionella spp |
| Applications | An ideal tool for a fast and reliable Amplification and Detection of specific DNA fragment from Legionella spp by the real-time PCR method. |
| Features & Benefits | • Reliable and highly specific Results. • Exclusivity: 100%. Primers and probes have been tested satisfactorily in 28 non-Legionella bacteria. • Simple and Fast: results in less than 24 hours with minimal handling. • Amplification limit: 2.5 copies/reaction (95%). • Thermal cycler: Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers. • Detection: probe labelled with fluorescent dyes– Legionella: FAM-TAMRA; IAC: JOE-TAMRA • Quantification limit: 5 copies/reaction (95%). • Easy and Ready-to-use Kit. • Inclusivity: 100%. Primers and probes have been tested satisfactorily in 29 strains of Legionella • Quantification Dynamic range: 8 logs |
| Kit Components | • PCR Positive Control • PCR Master Mix • PCR Negative Control |
| Storage Conditions | -20°C |
| Shipping Conditions | Dry Ice |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Legionella spp Detection Kit is an ideal tool for a fast and reliable Amplification and Detection of specific DNA fragment from Legionella spp by the real-time PCR method. The Kit includes all reagents required in a comfortable ready-to-use PCR Master Mix. The optimized Master Mix contains a Buffer, dNTPs, Hot-start DNA Polymerase, DNA-free water, MgCl2 and an Internal Amplification Control (IAC) whose detection indicates the absence of PCR inhibitors. Primers and Probes for the amplification of IAC as well as for the amplification of the target gene are included in the Master Mix. The Probe for the detection of Target Gene is labelled with the FAM (Legionella spp), whereas the probe for the detection of IAC is labelled with the JOE fluorochrome.
In addition, the kit includes both Positive Control and Negative Control. The Positive Control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNA ready Lysis Buffer to extract the DNA from the sample prior to PCR Detection.
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