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PARP (Cleaved) Antibody

based on 1 citations in multiple journalsPARP (Cleaved) Antibody14.1 4
Catalog #: 3140

In stock

$385.00

Product Details

Cat # +Size 3140-100
Size 100 µg
Antibody Target PARP
Host Rabbit
Antibody Type Polyclonal
Isotype Rabbit IgG
Immunogen Synthetic peptide correspond to the N-terminal residues of the catalytic domain of human PARP
Accession # NP_001609
Gene ID 142
Appearance Colorless liquid
Concentration 0.2 mg/ml
Formulation 100 µg (0.2 mg/ml) affinity purified rabbit polyclonal anti-cleaved PARP antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
Purification Affinity purified
Species Reactivity Human
Application Western blot, Immunohistochemistry
Application & Usage Western blot analysis (0.5-4 µg/ml) and immunocytochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes only the large fragment of PARP (89 kDa) and does not react with the full length PARP
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.


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Fernando, P., et al. (2002) PNAS 99:11025-11030.
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