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PARP-1, human recombinant

Involved in DNA repair and programmed cell death
Catalog #: 4992
$345.00

Product Details

Cat # +Size 4992-50
Size 50 μg
Alternate Name Human PARP, PARP, h-PARP, rh-PARP, recombinant human PARP, PARPrecombinant PARP, PARP
Gene Symbol PARP-1
Gene ID 142
Accession # P09874
Source Baculovirus
Appearance Lyophilized protein
Physical Form Description Lyophilized solid
Molecular Weight 116.0 kDa
Purity by SDS-PAGE ≥95%
Biological Activity 1000 U/vial, specific activity = 20000 U/mg PARP1. 1U=10 fmol ADP-ribose incorporated into 5 μg immobilized histone in 30 min at room temperature. Note: Activity measurements are approximate values.
Reconstitution Instructions Spin tube in a microfuge for 15 sec to sediment lyophilized material. Carefully open the vial and add 100 μL dH₂O. Vortex gently for 20 sec (avoid air bubbles). Let stand for 5 min. Carefully triturate the sample 10-times using a pipetman (avoid air bubbl
Handling Centrifuge the vial prior to opening.
Storage Conditions -70°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Poly-ADP-ribose metabolism plays a major role in a wide range of biological processes, such as maintenance of genomic stability, transcriptional regulation, energy metabolism and apoptosis. PARP1 Enzyme, is highly purified and enzymatically active human PARP1, expressed in a baculovirus expression system. Upon addition of NAD+ and cofactors the PARP1 is automodified by the addition of ADP-ribose monomers to form poly(ADP-ribose) polymer. The PARP1 is supplied lyophilized, and upon reconstitution with water, it retains its native poly ADP-ribosylation enzymatic activity. It is useful for high-throughput enzymatic assays, visualization of the automodification reaction by SDS-PAGE and Western blotting, ELISA, standard for SDS-PAGE, and in other assays.


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Are there any tags? If yes, where is it located?
Yes, there is a FLAG-tag located on the N-terminal
Would you please provide the protocol for testing the activity of 4992?
PARP1 enzyme activity was assayed by the incorporation of ADP-ribose into immobilized histone for 1 hr at RT. Conditions used in the reaction were PARP1 (15 ng/well) pre-incubated with increasing concentrations of 3-aminobenzamide performed in duplicates.
Is the protein tagged? Does it have a His-tag? How was the protein purified? Was it purified over a tag?
The PARP1 protein has a FLAG tag. There is no his-tag. The purification did not use any tag. The purification was done by ion exchange/conventional chromatography.