Product Details
| Cat # +Size | 3542-100 |
|---|---|
| Size | 100 µg |
| Antibody Target | p42/44 MAPK |
| Host | Rabbit |
| Antibody Type | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | Synthetic peptide from human p44 MAP kinase |
| Accession # | P27361 |
| Gene ID | 5595 |
| Appearance | Colorless liquid |
| Concentration | 0.5 mg/ml |
| Formulation | 100 µg (0.5 mg/ml) peptide affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal. |
| Purification | Affinity purified |
| Species Reactivity | Human, mouse, rat |
| Application | Western blot, Immunoprecipitation, Immunohistochemistry |
| Application & Usage | Western blotting (1 µg/ml), immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes p44/42 MAP kinase (Erk1/Erk2) of human, mouse, and rat or |
| Handling | The antibody solution should be gently mixed before use. |
| Storage Conditions | -20 °C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. Activation of MAP kinases occurs throμgh phosphorylation of threonine and tyrosine at the sequence T*EY* by a single upstream MAP kinase kinase (MEK).
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