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P-Cadherin (Human) ELISA Kit

A highly sensitive sandwich ELISA kit for the quantitative measurement of P-Cadherin

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5121
$750.00

Product Details

Cat # +Size E5121-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of P-Cadherin in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Sensitivity: 37.5 pg/ml
● Highly sensitive and specific
● Recovery Rate: 86-102% for serum, 91-105% for EDTA plasma and 85-95% for heparin plasma
● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
● Detection range: 62.5-4000 pg/ml
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (4000 pg)
● Sample/Standard Dilution Buffer
● Antibody Dilution Buffer
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

P-cadherin also known as Cadherin-3 or Placental cadherin, is a classical cell-to-cell adhesion molecule with a homeostatic function in several normal tissues. Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. Thus, cadherins contributes to the sorting of heterogeneous cell types. The cadherin family is essential in maintaining the cell-cell contact and regulate cytoskeletal complexes. The cadherin superfamily includes cadherins, protocadherins, desmogleins, desmocollins, etc. P-cadherin acts as a tumor suppressor, since its absence is associated with a more aggressive cancer cell phenotype. The overexpression of this molecule is linked to significant tumor promoting effects in the breast, ovarian, prostate, endometrial, skin, gastric, pancreas and colon neoplasms. P-cadherin acts as a pro-adhesive and anti-invasive/antimigratory molecule in colon carcinoma cells and stable knockdown in RT-112 cells diminishes invasion and migration, and thus promotes intercellular adhesion. BioVision’s P-Cadherin (Human) ELISA Kit is used for the quantitative detection of P-Cadherin in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of P-Cadherin in the sample.


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