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Oxytetracycline ELISA Kit

A Competitive ELISA kit for quantitative measurement of Oxytetracycline in tissue, milk, eggs, and feed samples

This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California
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Catalog #: E4957

Product Details

Cat # +Size E4957-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications Competitive ELISA kit to quantitatively measure Oxytetracycline in tissue, milk, egg and feed samples
Features & Benefits ● Sensitivity: 0.3ppb
● Detection Limit: 6ppb for muscle, milk, egg, 400ppb for feed
● Cross reaction: Oxytetracycline 100%, Chlortetracycline 300%, Tetracycline 206%, Doxycycline 18.5%
● This ELISA kit is used for in vitro quantitative determination of Oxytetracycline
Kit Components ● Micro ELISA Plate Standard (S0 – S5)
● HRP Conjugate
● Antibody working solution
● Substrate A
● Substrate B
● Stop Solution
● Wash Buffer (20X)
● Sample Diluent (20X)
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Oxytetracycline is a broad-spectrum antibiotic that belongs to the class of tetracyclines. It is effective against bacterial, chlamydial, and mycoplasma infections. The mechanism of action of this drug is to bind to 30S ribosomal subunit and prevent initiation of translation by binding of aminoacyl tRNA to the A site of the ribosome, thereby inhibiting protein synthesis. The drug can be used as a feed additive for food-producing animals to treat bacterial infections. It can also be used to promote growth in food-producing animals, promote milk and egg production and improve the efficiency of feed. BioVision’s Oxytetracycline ELISA kit is used to quantitatively measure Oxytetracycline in tissue, milk, eggs, and feed samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Oxytetracycline antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Oxytetracycline in the samples.

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