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Oxalate (Oxalic Acid) Colorimetric Assay Kit

based on 1 citations in multiple journalsOxalate (Oxalic Acid) Colorimetric Assay Kit14.1 4
Sensitive, High-Throughput adaptable
Catalog #: K663
$525.00

Product Details

Cat # +Size K663-100
Size 100 assays
Detection Method Absorbance (OD 450 nm)
Applications Measurement of Oxalate level in various samples.
Analysis of kidney and liver functions.
Features & Benefits • Simple, convenient and high throughput adaptable
• Kit can detect Oxalate levels lower than 20 µM.
Kit Components • Oxalate Assay Buffer
• Oxalate Development Buffer
• Oxalate Converter
• Oxalate Enzyme Mix (lyophilized)
• Oxalate Probe (lyophilized)
• Oxalate Standard (lyophilized)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Oxalate (C2O42-), in the form of Oxalic acid is present in many foods and beverages (e.g. spinach, tea etc.). It accumulates in many plant tissues and play role in regulating pH, osmosis and calcium storage. In animals, oxalate is either absorbed from dietary intake or produced from glycolate metabolism in liver. Under normal conditions, the daily oxalate load can be excreted by kidney. However, hereditary defects can cause an increased level of oxalate, which leads to hyperoxaluria, and results in the formation of kidney stones. Therefore, measurement of oxalate level is useful for the prevention, diagnosis and monitoring of kidney stones. BioVision’s Oxalate Assay kit is an easy-to-use, sensitive and high throughput adaptable kit. In this assay, Oxalate reacts with Oxalate Converter & Oxalate Enzyme Mix to form an intermediate, which in turn reacts with a highly specific probe to generate color at 450 nm. The assay kit can detect Oxalate levels lower than 20 µM.


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Can this kit be used with plant tissue?
Our Oxalic acid assay can work with a variety of samples as long as there is oxalic acid in the range of the std. curve. This kit can detect Oxalate levels lower than 20 µM. Please follow the protocol mention on the datasheet for plant tissues.
How does adding oxalate standard into the sample help?
Spiking a parallel contro sample with a known amount of oxalate std. helps account for any interference from endogenous sources in the sample. 4 ul of 1 mM Oxalate is 4 nmoles of oxalate and this amount should be recovered when the OD is read.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. It is recommended to do a pilot expt with multiple sample volumes to determine the optimum amount which gives a reading within the linear range of the standard curve.
Is it possible to use a different wavelength than recommended for the final analysis?
It is always recommended to use the exact recommended wavelength for the most efficient results. However, most plate readers have flexibility in their band width of detection in increments of +/- 10 nm. Depending on this flexibility range, you can deviate from the recommended wavelengths within limits.
Are trial sizes of this kit available?
Unfortunately, we do not have trial sizes of this kit available. However,for customers based in the US or Canada, a 10% off list price introductory discount can be offered on the list price. For all other customers based out of this area our regional BioVision distributor should be contacted.
What is the shelf life of this kit?
This kit is good for 12 months from the date of shipment in the unopened form when stored at the appropriate temperature and appropriate conditions. After opening and reconstitution, some of the components in this kit are good for 2 months at -20°C. Please refer to the datasheet for storage information and shelf life of each of the components.
Can individual components of this kit be purchased separately?
Yes, any of the kit's components can be purchased separately without having to buy the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can frozen samples be used with this assay?
Fresh samples are always preferred over frozen samples since this is an enzyme activity assay. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.
Alenzi, Mohammed et al. (2017) Antiurolithic effect of olive oil in a mouse model of ethylene glycol-induced urolithiasis, Investig Clin Urol. 2017 May;58(3):210-216.
For more citations of this product click here