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Osteopontin, human recombinant

Plays an important role in bone remodeling
Catalog #: 7335
$295.00

Product Details

Cat # +Size 7335-50
Size 50 μg
Alternate Name OPN, SPP-1, BNSP, BSPI, ETA-1
Gene Symbol SPP1
Gene ID 6696
Accession # P10451 
Source E. coli
Appearance Liquid
Physical Form Description 1 mg/ml in 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol, and 2 mM EDTA.
Molecular Weight 36.2 kDa (321 aa, 17-314 aa + His Tag), confirmed by MALDI-TOF. (MW on SDS-PAGE shifts up)
Purity by SDS-PAGE ≥90%
Endotoxin Level < 1.0 EU per 1 microgram of protein
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH RSMIPVKQAD SGSSEEKQLY NKYPDAVATW LNPDPSQKQN LLAPQNAVSS EETNDFKQET LPSKSNESHD HMDDMDDEDD DDHVDSQDSI DSNDSDDVDD TDDSHQSDES HHSDESDELV TDFPTDLPAT EVFTPVVPTV DTYDGRGDSV VYGLRSKSKK FRRPDIQYPD ATDEDITSHM ESEELNGAYK AIPVAQDLNA PSDWDSRGKD SY
Handling Centrifuge the vial prior to opening.
Storage Conditions -80°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not to be used in humans

Details

Osteopontin (OPN), also known as Secreted phosphoprotein 1 (SPP1), is a human gene product which is conserved in other species. This protein is glycoprotein that was first identified in osteoblasts and plays an important role in bone remodeling, immune functions in fibroblasts, macrophages, and lymphocytes during inflammation and wound healing. Recombinant human Osteopontin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.


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