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Ornithine Assay Kit (Fluorometric)

The first kit in the market that measures Ornithine in biological samples
Catalog #: K939

Product Details

Cat # +Size K939-100
Size 100 assays
Detection Method Fluorometric (Ex/Em = 535/587 nm)
Species Reactivity All
Applications Analysis of urea cycle and synthesis of polyamines,Measurement of Ornithine in biological samples
Features & Benefits • Kit contains the necessary reagents for accurate measurement of Ornithine
• Specific & Sensitive
• Simple procedure; takes ~ 120 minutes
• Fast and convenient
Kit Components • Ornithine Enzyme Mix
• Ornithine Converter Mix
• Ornithine Probe (in DMSO)
• Tissue Cleanup Mix
• Ornithine Standard
• Ornithine Assay Buffer
• Ornithine Developer Mix
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Ornithine is a non-standard, non-proteogenic amino acid. Ornithine is converted to putrescine via ornithine decarboxylase, which is the first step in the synthesis of polyamines. Additionally, Ornithine is one of the main metabolites that participate in the urea cycle in order to excrete excess nitrogen in the form of urea. In cells, Arginase hydrolyzes Arginine producing Ornithine and Urea (cytosol). Then, ornithine enters the mitochondria, reacts with carbamoyl phosphate forming Citrulline. Ornithine has therapeutics uses: it is administered to patients suffering brain complications caused by hepatic encephalopathy. Additionally, early research has suggested Ornithine may improve performance in some professional athletes. BioVision’s Ornithine Assay Kit provides a rapid, specific, and easy method for the measurement of total ornithine concentrations in a wide variety of biological samples. In this enzyme-based assay, ornithine is converted into a series of intermediates, which will further react with a probe producing a stable fluorometric signal (Ex/Em = 535/587nm). The kit is simple to use, sensitive and high-throughput adaptable and can detect as low as 50 pmol/well of ornithine in biological samples.

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Can I use the tissue cleanup mix included in the kit on my cell samples? Can you please provide sample preparation protocol for cells?
You can use the tissue clean up mix for your cells as well. Homogenize 1 x 106 cell in 100 μl of assay buffer provided with the kit. You can use either a dounce homogenizer or sonicate on ice to homogenize and lyse the cells. Centrifuge the lysate at 10,000 g for 10 min and take the supernatant. Add 2 μl of tissue clean up mix to 100 μl of supernatant. Incubate at 37˚C for 30 min. Transfer treated samples into a 10 kDa MWCO Spin Column (BV Cat# 1997). Centrifuge sample at 10,000 x g for 20 min at 4°C and collect the filtrate and use it for the assay.