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Omentin (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of Omentin in human plasma, tissue lysates and other biological fluids
Catalog #: E4880
$725.00

Product Details

Cat # +Size E4880-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This Sandwich ELISA kit is designed to quantitatively measure amount of Omentin in human serum, plasma, tissue lysates and other biological fluids
Features & Benefits • Sensitivity: 46.875 pg/ml
• This Sandwich ELISA is highly sensitive and highly specific for the detection of Omentin in human samples. There is no significant cross-reactivity or interference between Omentin and analogues
• Recovery range: 86 - 105% for normal human serum and plasma samples
• Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
• Detection range: 78.125 - 5000 pg/ml
Kit Components • Micro ELISA Plate
• Standard (Lyophilized) (5000 pg)
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate Solution
• Stop Solution
• Wash Buffer (25X)
• Plate Sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Omentin (Intelectin-1, ITLN1) is an adipokine of 313 amino acids that is secreted by the visceral fat adipose tissue and expressed in the adipose tissue stromal vascular cells, heart, ovary, lungs, and placenta. Omentin has been demonstrated to promote insulin-stimulated glucose uptake in human adipocytes. Lower levels of Omentin are associated with obesity-related metabolic disorders such as impaired glucose regulation, insulin resistance, and Type 2 Diabetes. Recent studies have demonstrated the role of Omentin in diseases such as cardiovascular disease, cancer, and inflammation. BioVision’s Omentin (Human) ELISA kit is designed to quantitatively measure the amount of Omentin in human serum, plasma, and other biological fluids. The kit is based on the sandwich ELISA principle. Test Samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and subsequently washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off with Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of Omentin in the sample or standard.


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