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OCT4 (OCT3) Antibody

Polyclonal Antibody to detect Oct4/Oct3 in human samples.
Catalog #: 6765
$265.00

Product Details

Cat # +Size 6765-100
Size 100 μl
Antibody Target Oct4/Oct3
Alternate Name POU5F1; OCT3; OCT4; OTF3; POU domain, class 5, transcription factor 1; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3
Host Rabbit
Antibody Type Polyclonal
Isotype Rabbit IgG
Immunogen This OCT4 (OCT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-141 amino acids from human OCT4 (OCT3).
Accession # Q01860
Gene ID 5460
Appearance Colorless liquid
Formulation Supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Species Reactivity Human.
Application Western blot, IF.
Positive Control Western blot: HL-60 cell lysate, IF: Sy5Y cells
Application & Usage Western blot: ~1:1000, IF: ~1:10–1:50.
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells. A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation. Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker. Oct-4 exists as two splice variants, Oct-4A and Oct-4B. Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells.


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