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Ochratoxin A (OTA) ELISA Kit

A Indirect-Competitive ELISA kit for the quantitative measurement of Ochratoxin A in grain and feed samples.
Catalog #: E4746

Product Details

Cat # +Size E4746-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications • Sensitivity: 0.1 ppb (ng/mL)
• Cross-reactivity: Ochratoxin A (OTA): 100%
• Detection limit: Grain: 1 ppb Feed: 2 ppb
• Sample recovery rate: Grain/Feed: 85% ± 15%
Features & Benefits • Cross-reactivity: Ochratoxin A (OTA): 100%
• Detection limit: Grain: 1 ppb Feed: 2 ppb
• Sample recovery rate: Grain/Feed: 85% ± 15%
• Sensitivity: 0.1 ppb (ng/mL)
Kit Components Substrate Reagent A
Stop Solution
Wash Buffer (20X)
Substrate Reagent B
ELISA Microplate
Standards (S1 - S6)
Antibody Working Solution
Plate Sealer
HRP Conjugate
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Ochratoxin A (OTA) is a mycotoxin produced by several fungal species including Aspergillus ochraceus, A. carbonarius, A. niger and Penicillium verrucosum. It is found as a contaminant of barley, corn, wheat, oats and coffee. OTA causes nephrotoxicity and renal tumors in a variety of animal species. BioVision OTA ELISA Kit is based on Indirect-Competitive-ELISA principal. It can detect Ochratoxin A (OTA) in samples, such as grain (rice, peanut, soybean, etc.) and feed. The micro ELISA plate provided in this kit has been pre-coated with OTA. During the reaction, OTA in the samples or standard competes with OTA on the solid phase supporter for sites of OTA antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of OTA. The concentration of OTA in the samples can be calculated by comparing the OD of the samples to the standard curve.

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