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NOS2 / iNOS (mouse) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of NOS2 in mouse serum, plasma, culture supernatants and other biological fluids.
Catalog #: E4691

In stock

$695.00

Product Details

Cat # +Size E4691-100
Size 96 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Mouse
• Application- quantitative measurement of NOS2 in serum, plasma, tissue homogenates and other biological fluids.
Detection Method colormetric
Species Reactivity Mouse
Applications For quantitative detection of NOS2/iNOS in serum, plasma, tissue homogenates and other biological fluids.,Sensitivity: < 0.188 ng/ml,Detection Range: 0.312 - 20 ng/ml
Features & Benefits • Sensitivity: < 0.188 ng/ml
• Detection method- Absorbance (450 nm)
• Detection Range: 0.312-20 ng/ml
• Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
• Application- quantitative measurement of NOS2 in serum, plasma, tissue homogenates and other biological fluids.
• No significant cross-reactivity or interference was observed
• Species reactivity- mouse
Kit Components • Antibody dilution buffer
• Wash buffer (25X)
• Sample / Standard dilution buffer
• HRP-Streptavidin Conjugate(SABC)
• SABC dilution buffer
• TMB substrate
• Micro ELISA Plate
• Plate Sealer
• Biotin-detection antibody (Concentrated)
• Stop solution
• Lyophilized Standard
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Nitric oxide synthases (NOS) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. In presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme, NOS catalyzes a five-electron oxidation of the guanidino nitrogen of L-arginine with molecular oxygen to generate NO and L-citrulline. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (iNOS). iNOS is expressed de novo under oxidative stress conditions and produces large amounts of NO as a part of body’s defense mechanism. BioVision’s NOS2 ELISA kit is a sandwich ELISA assay for the quantitative measurement of NOS2 in mouse serum, plasma and cell culture supernatants. The density of color is proportional to the amount of NOS2 captured from the samples.


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