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Norfloxacin ELISA Kit

A Competitive ELISA kit for in vitro quantitative determination of Norfloxacin concentrations in Tissue, Honey, Milk, Milk powder, Eggs samples.
Catalog #: E4776

Product Details

Cat # +Size E4776-100
Size 96 assay
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications In vitro quantitative determination of Norfloxacin concentrations in Tissue, Honey, Milk, Milk powder, Eggs samples.
Features & Benefits • Detection limit:Tissue (chicken, porcine, fish, shrimp) - 0.06 ppb, Honey - 0.08 ppb. Milk- 0.6 ppb, Milk powder- 2 ppb, Eggs- 0.9 ppb, Urine - 0.15ppb
• Sample recovery rate: Tissue, Honey, Milk, Milk powder, Eggs - 85%±15%.
• Cross-reactivity: Norfloxacin- 100%
• Sensitivity: 0.03 ppb (ng/mL)
Kit Components • ELISA Microplate
• Standard
• HRP Conjugate
• Antibody Working Solution
• Substrate Reagent A
• Plate Sealer
• Stop Solution
• Wash Buffer (20X)
• Reconstitution Buffer (5X)
• Substrate Reagent B
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Norfloxacin is an antibiotic that belongs to the class of fluoroquinolone antibiotics. It is used to treat urinary tract infections, gynecological infections, inflammation of the prostate gland, gonorrhea and bladder infection. Norfloxacin is associated with a number of rare serious adverse reactions as well as spontaneous tendon ruptures and irreversible peripheral neuropathy. Tendon problems may manifest long after therapy had been completed and in severe cases may result in lifelong disabilities. Norfloxacin ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with Norfloxacin. During the reaction, Norfloxacin in the samples or standard competes with Norfloxacin coated on the plate for binding to the anti-Norfloxacin antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Norfloxacin. The concentration of Norfloxacin in the samples can be calculated by comparing the OD of the samples to the standard curve.

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