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NLRP3 (Human) ELISA Kit

A Sandwich ELISA kit to quantitatively measure NLRP3 in human serum, plasma, tissue lysates, and other biological fluids
Catalog #: E4890
$695.00

Product Details

Cat # +Size E4890-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This Sandwich ELISA kit is designed to quantitatively measure amount of NLRP3 in human serum, plasma, tissue lysates, and other biological fluids
Features & Benefits • Detection range: 0.781 - 50 ng/ml
• This Sandwich ELISA is highly sensitive and highly specific for the detection of NLRP3 in human samples. There is no significant cross-reactivity or interference between NLRP3 and analogues
• Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
• Sensitivity: 0.469 ng/ml
• Recovery range: 85 - 105% for normal human serum and plasma samples
Kit Components • Micro ELISA plate
• Standard (Lyophilized) (50 ng)
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate Solution
• Stop Solution
• Wash Buffer (25X)
• Plate Sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Nod-like receptor protein 3 (NLRP3) is a 115 kD cytosolic protein that belongs to the NLR family. The protein is expressed in dendritic cells, neutrophils, and lymphocytes. NLRP3 is a component of the NLRP3 inflammasome complex that includes the NLRP3 scaffold, PYCARD (PYD and CARD Domain) adaptor, and caspase-1. Assembly of this complex results in caspase-1 dependent release of pro-inflammatory cytokines such as IL-1β and IL-18 and induces inflammation. Activation of the NLRP3 inflammasome complex is associated with several diseases such as atherosclerosis, type II diabetes, Alzheimer's disease (AD), chronic obstructive pulmonary disease, tumors, cryopyrin-associated periodic syndrome, and COVID-19 disease. Hence, NLRP3 could serve as an efficient therapeutic target for the treatment of human diseases. BioVision’s NLRP3 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of CHI3L1 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of NLRP3 in the sample or standard.


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