Product Details
| Cat # +Size | 5416-50 |
|---|---|
| Size | 50 µl |
| Antibody Target | Nitrotyrosine |
| Host | Rabbit |
| Antibody Type | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | Nitrated keyhole limpet hemocyanin (KLH). |
| Appearance | Provided as rabbit serum |
| Concentration | Undetermined in neat serum |
| Formulation | Supplied as rabbit serum. Does not contain sodium azide |
| Species Reactivity | All species |
| Application | Western blotting , ELISA. |
| Application & Usage | Western blotting 1/500 using alkaline phosphatase and BCIP/NBT, ELISA (Recognizes nitrated BSA 1/1,000,000 in ELISA). |
| Handling | The antibody solution should be gently mixed before use. |
| Storage Conditions | -20 °C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Nitrotyrosine is a product of tyrosine nitration mediated by reactive nitrogen species such as peroxynitrite anion and nitrogen dioxide. It is a marker for inflammation and nitric oxide (NO) production. It is detected in large number of pathological conditions and is considered a marker of NO-dependent, reactive nitrogen species-induced nitrative stress. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues for their activity. Nitration prevents phosphorylation of tyrosine residues important for signal transduction. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques, chronic renal failure and keratoconus. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine Antibody detects proteins and peptides containing nitro-tyrosine in a manner independent of the surrounding amino acid sequence. It is a valuable tool for identifying new nitrated proteins as well as for assaying protein nitration and measuring levels of nitrated proteins in tissues and samples.
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