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Nitrotyrosine Antibody (HM11)

Catalog #: 5414
$550.00

Product Details

Cat # +Size 5414-50
Size 50 µg
Antibody Target Nitrotyrosine
Host Mouse
Antibody Type Monoclonal
Isotype Mouse IgG1
Immunogen Nitrated keyhole limpet hemocyanin (KLH).
Appearance Colorless liquid
Concentration 0.5 mg/ml
Formulation Sterile, no preservatives added.
Species Reactivity All species
Application Western Blot, ELISA, Immunohistochemistry.
Application & Usage Western Blot (1 µg/ml) using secondary antibody coupled to alkaline phosphatase and BCIP/NBT as substrate, ELISA (1:1000), Immunohistochemistry.
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Nitrotyrosine is a product of tyrosine nitration mediated by reactive nitrogen species such as peroxynitrite anion and nitrogen dioxide. It is a marker for inflammation and nitric oxide (NO) production. It is detected in large number of pathological conditions and is considered a marker of NO-dependent, reactive nitrogen species-induced nitrative stress. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues for their activity. Nitration prevents phosphorylation of tyrosine residues important for signal transduction. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques, chronic renal failure and keratoconus. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine Antibody detects proteins and peptides containing nitro-tyrosine in a manner independent of the surrounding amino acid sequence. It is a valuable tool for identifying new nitrated proteins as well as for assaying protein nitration and measuring levels of nitrated proteins in tissues and samples.


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