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Nitrite Assay Kit (Griess Reagent)

Detects total nitrite
Catalog #: K544

In stock

SKU-Size Size Price Qty
K544-200 200 assays
$185.00
K544-500 500 assays
$335.00
K544-1000 1000 assays
$605.00
More Sizes Get Quote

Product Details

Kit Summary • Detection method- Absorbance (540 nm)
• Species reactivity- Mammalian
• Applications- BioVision’s Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of nitrite in a simple two-step process.
Detection Method Detection method- Absorbance (540 nm)
Species Reactivity Eukaryotes
Applications Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of nitrite in a simple two-step process.
Features & Benefits • Simple two-step procedure; takes only ~1-1.5 hours
• Fast and convenient
• BioVision’s Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of total nitrite.
Kit Components • Assay Buffer
• Griess Reagent R1
• Griess Reagent R2
• Nitrite Standard
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Nitrogen-based ions, nitrite and nitrate, are found in almost every living organism. Furthermore, endogenous Nitrite levels are found in mammals and also can be obtained from dietary sources. In humans, nitrite is further metabolized to Nitric Oxide and other reactive nitrogen species (nitrogen oxides). The Nitrate-Nitrite-NO biochemical pathway is well known for its participation in cell signaling, hypoxia-dependent response and regulation of blood flow. Recent studies suggest the reduction of Nitrite to Nitrogen Oxygen in the mitochondria. Specifically, myoglobin and xanthine oxidoreductase could generate NO under hypoxic conditions leading to mitochondrial respiration. BioVision’s Nitrite Assay Kit utilizes the Griess Reagent, a classic protocol for the estimation of nitrite. In the assay, nitrite is reduced to Nitrogen Oxide using Griess Reagent I. Then, Nitrogen Oxide reacts with Griess Reagent II forming a stable product that can be detected by its absorbance at OD 540 nm. The one-step assay is simple, fast and can detect nitrite levels as low as 1 nmol/well.


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