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Nicotinamide N-methyltransferase Inhibitor Screening Kit II (F)

For screening/studying/characterizing potential inhibitors of NNMT
Catalog #: K2081
$515.00

Product Details

Cat # +Size K2081-100
Size 100 assays
Kit Summary BioVision’s Nicotinamide N-methyltransferase inhibitor screening Kit II is a non-coupled, plate-based fluorometric assay for screening potential inhibitors of NNMT.
Detection Method Fluorescence (Ex/Em = 320/420 nm)
Species Reactivity Mammalian
Applications For screening, studying and characterizing potential inhibitors of NNMT
Features & Benefits ● A non-coupled, plate-based fluorometric assay.
● A one-step, rapid, reliable test for high-throughput screening of NNMT inhibitors.
Kit Components ● NNMT Assay Buffer
● DTT (1 M)
● NNMT Enzyme
● S-Adenosylmethionine (SAM)
● NNMT Substrate
● 1-Methylnicotinamide
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Nicotinamide N-methyltransferase (NNMT; EC 2.1.1.1) is an important cytosolic enzyme. It catalyzes the N-methylation of nicotinamide by transferring a methyl group from S-adenosyl-L-methionine (SAM) to nicotinamide resulting in the formation of 1-methyl-nicotinamide (MNA) and S-adenosyl-L-homocysteine. NNMT plays a significant role in the regulation of metabolic pathways in tissues such as adipose and liver as well as in cancer cells. Abnormal NNMT expression and activity has been implicated in a number of chronic diseases including cardiovascular diseases, cancer, obestity, diabetes, osteoarthritis, Parkinson’s disease etc. Therefore, small molecule inhibitors of NNMT could be beneficial in developing therapeutics for diseases characterized by abnormal NNMT activity. BioVision’s Nicotinamide N-methyltransferase inhibitor screening Kit II is a non-coupled, plate-based fluorometric assay. The assay utilizes a selective NNMT substrate that is methylated by SAM to generate a fluorescent product, measured at Ex/Em = 320/420 nm. In the presence of 1-Methylnicotinamide, a NNMT inhibitor, the enzymatic activity is inhibited thereby resulting in a reduced fluorescent signal. The kit provids a one-step, rapid, reliable test for high-throughput screening of NNMT inhibitors.


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