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NFkB RelA/p65 Transcription Factor Activity Assay Kit (Colorimetric)

A 96-well plate based colorimetric assay to measure the activation of human NFkB p65 in nuclear extracts or cell lysates.

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: K2093
$695.00

Product Details

Cat # +Size K2093-100
Size 100 assays
Kit Summary BioVision’s NFkB RelA/p65 Transcription Factor Activity Assay is a 96-well plate based colorimetric assay to measure the activation of human NFkB p65 in nuclear extracts or cell lysates.
Detection Method Absorbance at 450 nm
Species Reactivity Human
Applications Semi-quantitative measurement of activation of human NFkB p65 in nuclear extracts or cell lysates.
Features & Benefits The kit offers a easy, rapid, sensitive and non-radioactive way to measure the activation of human NFkB p65 in nuclear extracts or cell lysates.
Kit Components ● Plate Coated with DNA Probes
● Non-Competitor Oligo (20 pmole)
● TMB Substrate
● Stop Solution
● Positive Control
● Plate Sealing Film
● Binding Buffer (5X)
● DTT (100 mM)
● Protease Inhibitor Cocktail
● RelA/p65 Primary Antibody
● Antibody Diluent Buffer
● HRP Conjugate Stock
● Wash Buffer (10X)
● Competitor Oligo (20 pmole)
Storage Conditions Multiple Temperatures
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not For Use in Humans.

Details

Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) represents a family of inducible transcription factors, which regulates many biological processes including immune responses, cell growth, apoptosis etc. This family includes five structurally related members namely NF-κB1 (or p50), NF-κB2 (or p52), RelA (or p65), RelB and c-Rel, which are normally sequestered in the cytoplasm by a family of inhibitory proteins and are inactive. Upon stimulation, NF-κB proteins form homo- or heterodimers and are transported into the nucleus where they bind to specific DNA sequences of targeted genes and activate transcription. Traditionally, western blot is used to detect the expression of NFkB and electrophoretic mobility shift assay or reporter assays are used to measure the NFkB activity respectively. But some of these methods are time consuming, laborious, and require radioactivity. BioVision’s NFkB RelA/p65 Transcription Factor Activity Assay is a 96-well plate based colorimetric assay to measure the activation of human NFkB p65 in nuclear extracts or cell lysates. The kit offers easy, rapid, sensitive and non-radioactive way to detect the activation of transcription factors in samples. In this assay, double stranded DNA sequence containing the NFkB p65 consensus binding site is coated on the 96-well plate. Active NFkB p65 in the cell lysate or the nuclear extract binds to the oligonucleotides on the plate. After the addition of RelA/p65 primary antibody that recognizes the NFkB p65-oligonucleotide complex, a HRP-conjugated secondary antibody is added followed by the addition of TMB substrate and a color signal is developed, which is measured at 450 nm.


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