Trypsin Activity Assay Kit (Colorimetric) (ab102531)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
Overview
-
Product name
Trypsin Activity Assay Kit (Colorimetric)
See all Trypsin kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Enzyme activity -
Assay time
2h 00m -
Species reactivity
Reacts with: Mammals -
Product overview
Trypsin Activity Assay Kit (Colorimetric) ab102531 is simple and fast assay to quantify trypsin activity.
In the trypsin activity assay protocol, trypsin cleaves a substrate to generate p-nitroaniline (p-NA) which is detected at OD = 405 nm. Since the color intensity is proportional to p-NA content, trypsin activity can be accurately measured.
Trypsin activity assay protocol summary:
- add samples, positive control and standards to wells
- add chymotrypsin inhibitor to sample and positive control wells and incubate for 10 min at room temp
- add reaction mix
- analyze every 2-3 min for 1-2 hr with microplate reader in kinetic mode at 25ºC -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K771 Trypsin Activity Colorimetric Assay Kit. K771-100 is the same size as the 100 test size of ab102531.
-
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 2000 tests Assay Buffer I 1 x 25ml 20 x 25ml Chymotrypsin Inhibitor II 1 x 100 units 20 x 100 units pNA Standard II 1 x 400µl 20 x 400µl Trypsin Inhibitor 1 x 100µl 20 x 100µl Trypsin Positive Control 1 vial 20 vials Trypsin Substrate 1 x 200µl 20 x 200µl -
Research areas
-
Function
Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates. -
Involvement in disease
Defects in PRSS1 are a cause of hereditary pancreatitis (HPC) [MIM:167800]; also known as chronic pancreatitis (CP). HPC is an autosomal dominant disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsOccurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
-
Alternative names
- Alpha-trypsin chain 2
- Anionic trypsinogen
- Beta trypsin
see all
Associated products
-
Related Products
Images
-
p-NA standard curve measured in duplicates ((+/- SD), background subtracted) after 60 minutes incubation.
-
Example of standard curve obtained using ab102531, following assay protocol. The curve should be used as guideline only.
-
Trypsin levels were kinetically measured in commercially purchased trypsin (1x); background signal subtracted (duplicates +/- SD).
-
Example of Trypsin sample timeline obtained using ab102531
Datasheets and documents
-
SDS download
-
Datasheet download
References (13)
ab102531 has been referenced in 13 publications.
- Chen X et al. Identification of HPCAL1 as a specific autophagy receptor involved in ferroptosis. Autophagy 19:54-74 (2023). PubMed: 35403545
- Hung YT et al. Decreased nutrient digestibility due to viscosity is independent of the amount of dietary fibre fed to growing pigs. Br J Nutr 127:177-187 (2022). PubMed: 33706826
- Liu K et al. Trypsin-Mediated Sensitization to Ferroptosis Increases the Severity of Pancreatitis in Mice. Cell Mol Gastroenterol Hepatol 13:483-500 (2022). PubMed: 34562639
- Gyurcsó G et al. Herbivorous Juvenile Grass Carp (Ctenopharyngodon idella) Fed with Genetically Modified MON 810 and DAS-59122 Maize Varieties Containing Cry Toxins: Intestinal Histological, Developmental, and Immunological Investigations. Toxins (Basel) 14:N/A (2022). PubMed: 35202180
- Gui F et al. Trypsin activity governs increased susceptibility to pancreatitis in mice expressing human PRSS1R122H. J Clin Invest 130:189-202 (2020). PubMed: 31550238