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PicoProbe™ Threonine Assay Kit (Fluorometric)

March 2018

PicoProbe™ Threonine Assay Kit (Fluorometric)

FOR IMMEDIATE RELEASE

MILPITAS, Calif. - March 7, 2018 - PRLog -- Threonine is one of the 20 naturally-occuring amino acids used by all organisms in the biosynthesis of proteins and is an essential dietary amino acid for humans. Threonine residues in proteins are subject to post-translational phosphorylation, a critical regulatory and signal transduction mechanism. In addition to its role as a protein building block, threonine can serve as a precursor to other amino acids and metabolites, including glycine, isoleucine and acetyl-coenzyme A. Recent research has shown that threonine is a vital nutrient for embryonic stem cells and threonine metabolism (mediated by the enzyme threonine dehydrogenase) is required for unlimited self-renewal and maintenance of stem cell pluripotency. Dietary threonine deficiency, while rare, can result in breakdown of the intestinal mucosal lining (eventually triggering inflammatory bowel disorders), immunosuppression and a multitude of neuropsychiatric symptoms. BioVision's Threonine Assay Kit allows for highly sensitive quantification of L-Threonine levels in biological fluids and tissues. The assay is based on the selective, NAD+-coupled enzymatic metabolism of threonine, yielding an oxidized intermediate and NADH. A developer enzyme mixture utilizes the NADH generated to convert PicoProbe™ into a stable fluorophore (Ex/Em = 538/587 nm). The assay is not affected by physiological concentrations of other amino acids, is high-throughput adaptable and can detect threonine levels down to 2 μM in samples.



Figure: Estimation of total L-Threonine in pooled normal human plasma (10 μl), single donor off-the-clot human serum (5 μl) and pooled human CSF (10 μl). L-Threonine concentrations for plasma, serum and CSF samples were 93.3 ± 3.7 μM, 159.7 ± 8.4 μM and 70.9 ± 3.1 μM, respectively.

For detail information on this product , visit: https://www.biovision.com/threonine-assay-kit-fluorometri...


About BioVision:

BioVision, Inc. is a privately held Life Science company headquartered in the beautiful San Francisco Bay area. BioVision develops and offers a wide variety of products including assay kits, antibodies, recombinant proteins & enzymes, and other innovative research tools for studying Apoptosis, Metabolism, Cell Proliferation, Cellular Stress, Cell Damage and Repair, Diabetes, Obesity and Metabolic Syndrome, Stem Cell Biology, Gene Regulation, Signal Transduction, and more.


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