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Phosphoglycerate Mutase Activity Assay Kit (Colorimetric/Fluorometric)

August 2019

Phosphoglycerate Mutase Activity Assay Kit (Colorimetric/Fluorometric)

To detect Phosphoglycerate Mutase (PGAM) activity in biological samples.

FOR IMMEDIATE RELEASE


MILPITAS, Calif. - Aug. 22, 2019 - PRLog -- Phosphoglycerate Mutase (PGAM) is an important enzyme in the glycolytic pathway that catalyzes the reversible conversion of 3‐ phosphoglycerate to 2‐phosphoglycerate. It plays an important role in regulating glycolysis and anabolic activity to promote cancer cell proliferation. Several studies reported that PGAM is upregulated in many human cancers. Inhibition of this enzyme could lead to tumor cell death thereby making PGAM an attractive therapeutic target. BioVision's PGAM Activity Assay Kit can be used to detect its activity in biological samples. The kit utilizes the ability of an active PGAM to catalyze the conversion of 3-Phosphoglycerate to 2-Phosphoglycerate, which is subsequently used to generate an intermediate product. The intermediate product then stoichiometrically reacts with an OxiRed™ probe to generate color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm). This assay kit provides a rapid, simple and sensitive method to detect PGAM activity as low as 0.05 mU in a variety of samples.

Figure: PGAM activity in rat liver lysate (0.2 µg), HepG2 lysate (0.4 µg) and Positive Control. Assays were performed following the kit protocol.

Get more information on this assayby visiting: https://www.biovision.com/phosphoglycerate-mutase-activity-assay-kit-colorimetric-fluorometric.html

About BioVision:
BioVision, Inc. is a privately held Life Science company headquartered in the beautiful San Francisco Bay area. BioVision develops and offers a wide variety of products including assay kits, antibodies, recombinant proteins & enzymes, and other innovative research tools for studying Apoptosis, Metabolism, Cell Proliferation, Cellular Stress, Cell Damage and Repair, Diabetes, Obesity and Metabolic Syndrome, Stem Cell Biology, Gene Regulation, Signal Transduction, and more.


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