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Oxidative Stress Assays

September 2018


Oxidative stress is a hallmark of a number of diseases including Alzheimer’s disease, Parkinson’s disease, Sickle cell disease, heart failure, myocardial infarction, Schizophrenia, fragile X syndrome, diabetes, cancer, cardiovascular disease and chronic fatigue syndrome. These pathological changes in the cells primarily occur due to DNA damage, oxidation of amino acids, proteins, polyunsaturated fatty acids and enzyme cofactors which subsequently causes cell death due to apoptosis or necrosis.

Key Features:
• Non-radioactive, versatile assays (Microplate)
• Specific, homogeneous assay
• Sensitive: colorimetric/fluorometric format
• Convenient: minimal sample preparation, fast protocols (< 2 hours)
• Cost effective: 100 assays; High Throughput Screening (HTS) compatible
• Adaptable: works with most of the commonly available instrumentations


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Description

K251: Glutathione Fluorometric Assay Kit

BioVision’s ApoGSH™ Glutathione Detection Kit provides a simple in vitro assay for detection of total glutathione changes in apoptosis and other samples. The assay utilizes monochlorobimane (MCB), a dye that appears to form an adduct exclusively with glutathione.

K252: Nitric Oxide Fluorometric Assay Kit

BioVision’s ApoGSH™ Glutathione Detection Kit provides a simple in vitro assay for detection of total glutathione changes in apoptosis and other samples. The assay utilizes monochlorobimane (MCB), a dye that appears to form an adduct exclusively with glutathione.

K253: DNA Damage Quantification Colorimetric Kit

Apurinic/apyrimidinic (AP) sites are one of the major types of DNA lesions formed duringthe course of base excision and repair of oxidized, deaminated or alkylated bases. It hasbeen estimated that about 2x105 base lesions are generated per cell per day.

K260: GST Fluorometric Activity Assay Kit

Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics.

K261: Glutathione Colorimetric Assay Kit

BioVision’s ApoGSH™ Glutathione Colorimetric Assay Kit provides a convenient, colorimetric method for analyzing either total glutathione or the reduced form glutathione alone using a microtiter plate reader.

K262: Nitric Oxide Colorimetric Assay Kit

BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process.

K263: GST Colorimetric Activity Assay Kit

Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics.

K264: Glutathione (GSH/GSSG/Total) Fluorometric Assay Kit

BioVision’s Glutathione Detection Kit provides a unique, convenient tool for detecting GSH, GSSG, and total glutathione individually. In the assay, OPA, reacts with GSH (not GSSG), generating fluorescence, so GSH can be specifically quantified.

K265: Hydrogen Peroxide Colorimetric/Fluorometric Assay Kit

BioVision’s Hydrogen Peroxide Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring H₂O₂ in biological samples.

K274: Total Antioxidant Capacity (TAC) Colorimetric Assay Kit

BioVision developed the TAC Assay Kit, which can measure either the combination of both small molecule antioxidants and proteins or small molecules alone in the presence of our proprietary Protein Mask.

K320: Senescence Detection Kit

The Senescence detection kit is designed to histochemically detect SA-β-Gal activity in cultured cells and tissue sections, a known characteristic of senescent cells. The SA-β-Gal is present only in senescent cells and is not found in presenescent, quiescent or immortal cells.

K330: HDAC Activity Fluorometric Assay Kit

The Fluorometric HDAC Activity Assay Kit provides a fast and fluorescence-based method that eliminates radioactivity, extractions, or chromatography, as used in traditional assays.

K331: HDAC Activity Colorimetric Assay Kit

The new Colorimetric HDAC Activity Assay Kit provides a fast and convenient colorimetric method that eliminates radioactivity, extractions, or chromatography, as used in the traditional assays.

K332: HAT Activity Colorimetric Assay Kit

BioVision’s HAT Activity Colorimetric Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active Nuclear Extract (NE) as a positive control and acetyl-CoA as a cofactor.

K335: Superoxide Dismutase (SOD) Activity Colorimetric Assay Kit

Superoxide dismutase (SOD) is one of the most important antioxidative enzymes. It catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen.

K340: HDAC Inhibitor Drug Screening Kit (Fluorometric)

Inhibition of histone deacetylase (HDAC) has been implicated to modulate transcription and to induce apoptosis or differentiation in cancer cells.

K608: Uric Acid Colorimetric/Fluorometric Assay Kit

BioVision’s Uric Acid Assay Kit provides a convenient means for detecting uric acid in biological samples such as serum and urine.

K620: Ethanol Colorimetric/Fluorometric Assay Kit

BioVision’s Ethanol Assay Kit provides a simple, rapid, and sensitive method for accurate quantification of ethanol concentration in a variety of biological samples such as serum, plasma, other body fluids, foods, beverages and growth media.

K629: Glutamate Colorimetric Assay Kit

BioVision’s Glutamate Assay Kit provides a sensitive detection method of the glutamate in a variety of samples.

K661: Ascorbic Acid Colorimetric/Fluorometric Assay Kit

BioVision’s Ascorbic Acid Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in various biological samples.

K671: Ascorbic Acid Colorimetric Assay Kit II (FRASC)

BioVision’s FRASC Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in biological samples such as serum and other body fluids, tissue and cell extracts, growth media and food products.

K710: Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit

BioVision has developed an easy and sensitive assay to determine XO in variety of samples. In the assay, XO oxidizes xanthine to hydrogen peroxide (H₂O₂) which reacts stoichiometrically with OxiRed™ Probe to generate color (at λ = 570 nm) and fluorescence (at Ex/Em = 535/587 nm).

K716: Aconitase Activity Colorimetric Assay Kit

BioVision’s Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples in less than 40 minutes.

K739: Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit

BioVision’s Lipid Peroxidation Assay Kit provides a convenient tool for sensitive detection of the MDA in a variety of samples. The MDA in the sample is reacted with Thiobarbituric Acid (TBA) to generate the MDA-TBA adduct.

K744: Myeloperoxidase (MPO) Colorimetric Activity Assay Kit

BioVision’s Myeloperoxidase (MPO) Colorimetric Activity Assay Kit, the HClO produced from H₂O₂ and Cl- is reacted with taurine to generate the taurine chloramine, which subsequently reacts with the TNB2- probe to eliminate color (λ = 412 nm).

K745: Myeloperoxidase (MPO) Fluorometric Activity Assay Kit

BioVision’s Myeloperoxidase (MPO) Fluorometric Activity Assay Kit, MPO catalyzes the production of NaClO from H₂O₂ and NaCl.

K761: Glutathione Reductase Activity Colorimetric Assay Kit

BioVision’s Glutathione Reductase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples.

K762: Glutathione Peroxidase Activity Colorimetric Assay Kit

BioVision’s Glutathione Peroxidase Assay Kit measures glutathione peroxidase (GPx) activity through a coupled reaction with glutathione reductase (GR).

K763: Thioredoxin Reductase Activity Colorimetric Assay Kit

BioVision’s Thioredoxin Reductase Assay Kit provides a convenient colorimetric assay for detecting TrxR activity in various samples.

K773: Catalase Activity Colorimetric/Fluorometric Assay Kit

BioVision’s Catalase Assay Kit provides a highly sensitive, simple, direct and HTS-ready assay for measuring Catalase activity in biological samples.

K991: Senescence Detection Kit (Fluorometric)

The Senescence detection kit is designed to fluorescently detect SA-β-Gal activity in cultured cells by FACS. The SA-β-Gal is present only in senescent cells and is not found in pre-senescent, quiescent or immortal cells.

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