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Methionine Adenosyltransferase Activity Assay Kit (Colorimetric)

March 2020

Methionine Adenosyltransferase Activity Assay Kit (Colorimetric)

Assay kit for the measurement of MAT activity in complex biological matrices


MILPITAS, Calif. - Mar. 13, 2020 - PRLog -- Methionine Adenosyltransferases (MATs, EC, also known as Adenosylmethionine Synthetases are a family of enzymes that synthesize S-adenosyl-L-methionine (SAMe) from L-methionine and ATP. SAMe is a primary biochemical alkylation agent (one of only two possible methyl group donors) and is also a vital metabolic precursor of the trans-sulfuration and polyamine synthesis (aminopropylation) pathways. All organisms express at least one MAT enzyme. In mammals, three isozymes of MAT have been identified. MAT1 and MAT3 isozymes are predominantly expressed in the liver, whereas MAT2A is expressed in most tissues. Upregulation of the MAT2A isozyme has been linked to several human diseases. MAT2A has become a popular drug target for novel cancer therapeutics as well as for hepatic fibrosis and non-alcoholic fatty liver disease. BioVision's Methionine Adenosyltransferase Activity Assay Kit enables the rapid measurement of MAT activity in complex biological matrices. The assay is based on the detection of pyrophosphate, which is generated stoichiometrically during the generation of SAMe. Pyrophosphate is enzymatically metabolized to an intermediate product, which reacts with the probe to form a stable chromophore that is detected by absorbance at 570 nm. The assay is homogeneous, simple to perform and does not require complicated sample processing. The assay is high-throughput adaptable and has a limit of quantification of 2 mU MAT activity per well.

Figure: (a) Pyrophosphate Standard Curve. One mole of Pyrophosphate Standard corresponds to the synthesis of one mole of SAMe, with release of one mole free pyrophosphate. (b) Reaction kinetics of MAT activity in homogenates of rat liver (0.8 µg protein/well), rat brain (0.8 µg protein/well) and HepG2 cell lysate (10 µg protein/well). (c) Quantification of MAT activity in Samples (mean ± SEM of 4 or more independent replicates). Assays were performed according to the kit protocol.

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BioVision, Inc. is a privately held Life Science company headquartered in the beautiful San Francisco Bay area. BioVision develops and offers a wide variety of products including assay kits, antibodies, recombinant proteins & enzymes, and other innovative research tools for studying Apoptosis, Metabolism, Cell Proliferation, Cellular Stress, Cell Damage and Repair, Diabetes, Obesity and Metabolic Syndrome, Stem Cell Biology, Gene Regulation, Signal Transduction, and more.

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