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Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric)

September 2018

Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric)


MILPITAS, Calif. - Sept. 20, 2018 - PRLog -- Lysophosphatidylcholine (LPC), also referred to as lysolecithin, is a phospholipid intermediate whose concentrations has been correlated with diseases like cancer, artherosclerosis and diabetes. Generally produced through the action of phospholipases on phosphatidylcholine, LPC consists of a glycerol backbone with a phosphocholine at one hydroxyl group and a single acyl chain attached to either the 1- or 2- position hydroxyl of the glycerol moiety. LPC is typically at concentrations in the high micromolar range in human serum and plasma, and is capable of activating second messengers such as Ca2+ and cyclic AMP. It is through these pathways that LPC has been found to affect such biological events as the pro-inflammatory response and intestinal uptake. BioVision's Lysophosphatidylcholine Assay Kit utilizes LPC-specific enzymes to generate an intermediate that then reacts with a probe, yielding a signal that can be quantified either colorimetrically or fluorometrically, and is proportional to the amount of LPC present in the sample. When used as described, the assay is capable of detecting as little as 10 pmole of lysophosphatidylcholine.

Figure: (A) Determination of total LPC concentration in pooled human serum. Sample was analyzed according to the kit protocol.  (B) Specificity of the assay; PC = phosphatidylcholine, PA = phosphatidic acid, SM = sphingomyelin, LPC = lysophosphatidylcholine;1 nmole of each was tested in the fluorometric assay.
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BioVision, Inc. is a privately held Life Science company headquartered in the beautiful San Francisco Bay area. BioVision develops and offers a wide variety of products including assay kits, antibodies, recombinant proteins & enzymes, and other innovative research tools for studying Apoptosis, Metabolism, Cell Proliferation, Cellular Stress, Cell Damage and Repair, Diabetes, Obesity and Metabolic Syndrome, Stem Cell Biology, Gene Regulation, Signal Transduction, and more.

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