Lipids Assays

March 2018  |  Metabolism Assays

Lipids


Lipids are often referred as the collection of “fat” molecules that can be endogenous or exogenous. They have essential functions and roles in humans from energy storage to cell signaling, to name a few. BioVision is proud to offer a complete portfolio to study lipid-related research. Our assay kits can be used to elucidate processes governing cellular biology, and cardiovascular-disease research.

Key Features:
• Non-radioactive, homogeneous assays
• Specific assays
• Versatile: spectrophotometry, fluorescence microscopy, cell-based assays
• Convenient: minimal sample preparation; fast protocols (1-2 hours )
• Cost effective: 50/100 assays; High Throughput Screening compatible
• Validated: using mammalian tissues, cells, biological fluids

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Description

K209: Total Bile Acids (TBA) Assay Kit (Colorimetric)

BioVision’s Total Bile Acid Assay Kit provides a simple, sensitive, and high-throughput adaptable approach to detect physiological concentration of total bile acids in a variety of biological fluids.

K582: Cholesterol Efflux Fluorometric Assay Kit (cell-based)

A negative correlation has been established between the in vitro to in vitro efflux of cholesterol from macrophages and atherosclerosis.BioVision’s Cholesterol Efflux Assay is a high-throughput screening assay for measuring cholesterol efflux in cells using fluorescently-labeled cholesterol. This assay provides a safe, sensitive, and reproducible method for measuring cholesterol efflux.

K586: HDL Uptake Assay Kit (Fluorometric)

BioVision’s HDL Uptake Assay Kit contains a fluorescently-labeled HDL that can be measured when taken up by cells. Unlabeled-HDL is included in the kit for assay validation.

K587: Cholesterol Detection Kit (cell-based)

This assay provides a simple, easy to perform, histochemical method of identification of unesterified cholesterol.

K600: Sphingomyelin Quantification Colorimetric Assay Kit

BioVision’s Sphingomyelin Quantification Assay Kit provides a simple and sensitive method for quantifying sphingomyelin using colorimetry.

K603: Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit

The assay detects total cholesterol (cholesterol and cholesteryl esters) in the presence of cholesterol esterase or free cholesterol in the absence of cholesterol esterase in the reaction.

K612: Free Fatty Acid Quantification Colorimetric/Fluorometric Kit

BioVision’s Free Fatty Acid Quantification Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids in various biological samples.

K613: HDL and LDL/VLDL Quantification Colorimetric/Fluorometric Kit

BioVision’s Free Fatty Acid Quantification Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids in various biological samples.

K614: PicoProbe™ Triglyceride (TG) Fluorometric Assay Kit

Biovision’s PicoProbe™ Triglyceride Assay kit is suitable for measuring triglyceride levels in samples, which contain reducing substances that may interfere with oxidase-based assays.

K622: Triglyceride Quantification Colorimetric/Fluorometric Kit

The Triglyceride (TG) Quantification Kit provides a sensitive, easy assay to measure TG concentration in a variety of samples.

K623: Total Cholesterol and Cholesteryl Ester Colorimetric Assay Kit II

In the assay, free cholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at 450 nm. The assay can detect free or total cholesterol.

K630: Free Glycerol Colorimetric/Fluorometric Assay Kit

BioVision’s Glycerol Assay Kit provides a sensitive, easy assay to measure free glycerol concentration in various samples.

K632: β-Hydroxybutyrate (β-HB) Colorimetric Assay Kit

Diabetic ketoacidosis occurs when circulating insulin levels drop to very low levels, shutting off the supply of glucose to the body.

K643: PicoProbe™ Free Glycerol Fluorometric Assay Kit

Biovision’s PicoProbe™ Free Glycerol Fluorometric Assay kit is simple, sensitive and easy to use. This assay is suitable for measuring trace amount of glycerol in samples, which contain reducing substances that may interfere with oxidase-based assays.

K650: Acetoacetate Colorimetric Assay Kit

BioVision’s Acetoacetate Assay Kit has adapted the principle with a modification that provides a sensitive method to quantitate endogenous levels of AcAc in human blood, and urine.

K651: PicoProbe™ β-Hydroxybutyrate Fluorometric Assay Kit

BioVision’s β-Hydroxybutyrate Assay Kit offers simplicity, enhanced sensitivity, and can be adapted to high-throughput applications. The assay is based on enzymatic oxidation of β-HB that results in generation of fluorescent signal which is directly proportional to the amount of β-HB.

K944: Cardiolipin Assay Kit (Fluorometric)

BioVision’s Cardiolipin Assay is a fluorometric assay that makes use of a proprietary probe that fluoresces on association with cardiolipin but not with any other lipids such as phosphatidylcholine and sphingomyelin, making it highly specific.

K954: EZScreen™ Beta-Lactamase Activity Colorimetric Assay Kit (384-well)

BioVision’s EZScreen™ Beta-Lactamase Activity Assay Kit offers a simple and sensitive assay that can detect and quantify the enzymatic activity of these hydrolases. The assay is based on the hydrolysis of Nitrocefin, a chromogenic cephalosporin, that results in the generation of a colored product.

K957: EZScreen™ Total Cholesterol & Cholesteryl Ester Colorimetric Assay Kit (384-Well)

The EZScreen™ assay detects total cholesterol (cholesterol and cholesteryl esters) in the presence of cholesterol esterase or free cholesterol in the absence of cholesterol esterase.

K977:Glycerol Cell-Based Assay Kit

BioVision’s Glycerol Cell-Based Assay Kit provides a simple, sensitive, straight-forward assay for determination of glycerol concentrations in various cell and tissue culture samples.