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Krebs Cycle Assays

March 2018

Krebs Cycle

The Krebs Cycle, also known as Tricarboxylic Acid (TCA), is considered one of the central pathwasy in cellular metabolism. It oxidizes several metabolites containing acetyl groups. The list includes amino acids, carbohydrates and fatty acids. BioVision offers the most complete series of assays measuring various metabolites, cofactors, and enzymes involved in the glycolytic pathway.


Key Features:
• Non-radioactive, homogeneous assays
• Specific assays
• Convenient: minimal sample preparation; fast protocols (1-2 hours )
• Cost effective: 100 assays; High Throughput Screening compatible
• Validated: using mammalian tissues, cells, biological fluids



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Description

PicoProbe™Acetyl-CoA Fluorometric Assay Kit

K317: PicoProbe™Acetyl-CoA Fluorometric Assay Kit

BioVision has developed a highly sensitive assay for determining Acetyl CoA level in a variety of biological samples.

Citrate Synthase Activity Colorimetric Assay Kit

K318: Citrate Synthase Activity Colorimetric Assay Kit

BioVision’s Citrate Synthase Activity Assay Kit, Citrate Synthase reacts with substrate mix to form an intermediate, which subsequently reacts with developer to generate the colored product.

NAD/NADH Quantitation Colorimetric Kit

K337: NAD/NADH Quantitation Colorimetric Kit

BioVision’s NADH/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio.

NADP/NADPH Quantitation Colorimetric Kit

K347: NADP/NADPH Quantitation Colorimetric Kit

The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio.

FAD Colorimetric/Fluorometric Assay Kit

K357: FAD Colorimetric/Fluorometric Assay Kit

BioVision’s FAD Assay Kit, FAD functions as the cofactor of an oxidase which catalyze the formation of a product that reacts with OxiRed probe generating color and fluorescence.

Phosphate Colorimetric Assay Kit

K410: Phosphate Colorimetric Assay Kit

The Phosphate Colorimetric Assay Kit provides an easy, quick and sensitive means of assessing phosphate over a wide range of concentrations. The assay utilizes a proprietary formulation of malachite green and ammonium molybdate.

Phosphate Assay Kit (Fluorometric)

K420: Phosphate Assay Kit (Fluorometric)

This Phosphate Assay Kit provides a sensitive, easy and quick means of assessing Pi over a wide range of concentrations. In this assay, inorganic phosphate reacts with sucrose to produce glucose-1-phosphate in the presence of a proprietary enzyme.

Fumarase Activity Colorimetric Assay Kit

K596: Fumarase Activity Colorimetric Assay Kit

Biovision’s Fumarase Activity Assay kit provides a quick and easy method for monitoring fumarase activity in various samples. In this kit, fumarase converts fumarate into malate, which then reacts with Enzyme Mix to form an intermediate.

Succinyl-CoA Synthetase Activity Colorimetric Assay Kit

K597: Succinyl-CoA Synthetase Activity Colorimetric Assay Kit

BioVision’s Succinyl-CoA Synthetase Activity Assay, SCS converts succinate into succinyl-CoA in the presence of ATP and CoA.

Fumarate Colorimetric Assay Kit

K633: Fumarate Colorimetric Assay Kit

BioVision’s Fumarate Assay Kit provides a convenient tool for sensitive detection of the fumarate in a variety of samples.

Malate Colorimetric Assay Kit

K637: Malate Colorimetric Assay Kit

BioVision has developed an easy and sensitive assay to measure the L(-) Malate level in a variety of samples.

Succinate (Succinic Acid) Colorimetric Assay Kit

K649: Succinate (Succinic Acid) Colorimetric Assay Kit

BioVision’s Succinate (Succinic Acid) Assay kit is a sensitive, fast and easy-to-use kit. In this assay, Succinate is utilized by Succinyl-CoA Synthetase to form an intermediate, which undergoes a series of reactions & reduces a colorless probe to a colored product with strong absorbance at 450 nm.

Malate Dehydrogenase Activity Colorimetric Assay Kit

K654: Malate Dehydrogenase Activity Colorimetric Assay Kit

BioVision’s Malate Dehydrogenase Activity Assay kit, MDH reacts with malate to form an intermediate.

Citrate Colorimetric/Fluorometric Assay Kit

K655: Citrate Colorimetric/Fluorometric Assay Kit

BioVision's Citrate Assay Kit provides a simple, sensitive and rapid means of quantifying citrate in a variety of samples. In the assay, citrate is converted to pyruvate via oxaloacetate.

Isocitrate Colorimetric Assay Kit

K656: Isocitrate Colorimetric Assay Kit

BioVision's Isocitrate Assay Kit provides a simple, sensitive and rapid means of quantifying isocitrate in a variety of samples. In the assay, isocitrate is oxidized with the generation of NADPH which converts a nearly colorless probe to an intensely colored species with a λmax of 450nm.

Oxaloacetate Colorimetric/Fluorometric Assay Kit

K659: Oxaloacetate Colorimetric/Fluorometric Assay Kit

BioVision's Oxaloacetate Assay Kit provides a simple, sensitive and rapid means of quantifying OAA in a variety of samples. In the assay, OAA is converted to pyruvate which is utilized to convert a nearly colorless probe to an intensely colored and fluorescent product.

Succinate Dehydrogenase Activity Colorimetric Assay Kit

K660: Succinate Dehydrogenase Activity Colorimetric Assay Kit

BioVision’s Succinate Dehydrogenase Activity Assay kit is rapid, simple and high-throughput adaptable. In this assay, Succinate dehydrogenase converts succinate to fumarate, and transfers the electron to an artificial electron acceptor, which changes the color from blue to a colorless product.

Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit

K677: Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit

BioVision's α-Ketoglutarate Assay Kit provides a simple, sensitive and rapid means for quantifying α-KG in a variety of samples. In the assay, α-ketoglutarate is transaminated with the generation of pyruvate which is utilized to convert a nearly colorless probe to both color and fluorescence.

Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit

K678: Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit

BioVision’s α-KGDH assay kit provides a quick and easy way for monitoring α-KGDH activity in various samples. In the assay, α-KGDH converts α-ketoglutarate into an intermediate which reduces the probe to a colored product with strong absorbance at 450 nm.

Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit

K679: Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit

BioVision’s PDH assay kit provides a quick and easy way for monitoring PDH activity in various samples. In the assay, PDH converts pyruvate into an intermediate, which reduces the developer to a colored product with strong absorbance at 450 nm.

Aconitase Activity Colorimetric Assay Kit

K716: Aconitase Activity Colorimetric Assay Kit

BioVision’s Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples.

Isocitrate Dehydrogenase Activity Colorimetric Assay Kit

K756: Isocitrate Dehydrogenase Activity Colorimetric Assay Kit

In BioVision’s ALT Assay Kit, ALT catalyzes the transfer of an amino group from alanine to ⍺-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate.

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