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Homocysteine Lyase Activity Assay Kit (Fluorometric)

December 2019

Homocysteine Lyase Activity Assay Kit (Fluorometric)

Sensitive method to assay HCYase activity in bacterial samples


MILPITAS, Calif. - Dec. 11, 2019 - PRLog -- Homocysteine α,γ-Lyase (HCYase) is a pyridoxal 5'-phosphate dependent enzyme that catalyzes the breakdown of homocysteine to hydrogen sulfide, ammonia, and α-ketobutyrate. HCYase is present in high activity in the anaerobic protozoan parasite Trichomonas vaginalis but is absent from mammals. The Trichomonas HCYase is highly selective for homocysteine as compared to cysteine. Additionally, it has broad pH stability (pH 6-8) and is thermally stable up to 50°C. This makes rHCYase a promising candidate for therapeutic applications including the diagnosis of hyperhomocystenemia, which has been demonstrated to be a major risk factor in cardiovascular diseases. BioVision's Homocysteine Lyase Activity Assay Kit is a simple, quick and sensitive method to assay HCYase activity in bacterial samples. The assay relies on the HCYase-catalyzed release of hydrogen sulfide (H2S), which reacts with a fluorogenic probe to form a stable fluorophore measured at Ex/Em = 368/460 nm.

Figure: Estimation of HCYase activity in lysates of wild type E. coli (BL-21) and rHCY expressing E. coli. Both lysates were spiked with 1.6 µU of Positive Control. The average spiked recoveries were 90.9% and 104.1% respectively. The assay was performed according to the kit K2026 assay protocol.

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About BioVision:

BioVision, Inc. is a privately held Life Science company headquartered in the beautiful San Francisco Bay area. BioVision develops and offers a wide variety of products including assay kits, antibodies, recombinant proteins & enzymes, and other innovative research tools for studying Apoptosis, Metabolism, Cell Proliferation, Cellular Stress, Cell Damage and Repair, Diabetes, Obesity and Metabolic Syndrome, Stem Cell Biology, Gene Regulation, Signal Transduction, and more.

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