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Glycoprotein Staining Assay Kit

February 2021

Glycoprotein Staining Assay Kit

For the staining and detection of glycoproteins on a PVDF or nitrocellulose membrane.


MILPITAS, Calif. - Feb. 22, 2021 - PRLog -- BioVision's Glycoprotein Staining Kit is designed for the staining and detection of glycoproteins on a PVDF or nitrocellulose membrane using the periodic acid-Schiff (PAS) method. The kit provides all the necessary reagents needed to specifically stain the glycoproteins separated by polyacrylamide gel electrophoresis and transferred to a nitrocellulose or PVDF membrane. The glycoproteins are detected as magenta bands against a colorless background. The kit contains sufficient reagents for staining up to 20 membranes (6 x 9 cm) and can detect as low as 0.5 µg of native glycoproteins.

Figures A. SDS-PAGE and Coomassie staining of various proteins. B. Selective staining of glycoproteins. Lanes, M: Protein Marker; 1: Deglycosylated recombinant glycoprotein; 2: Recombinant glycoprotein; 3: Positive control (native glycoprotein); 4: Negative control. C. Glycoprotein staining using various amounts of native glycoprotein (10, 5, 2.5, 1 and 0.5 µg respectively). For deglycosylation, 10 µg of glycoprotein (BioVision Cat# P1172) was treated with 0.1 mU of PNGase F (BioVision Cat# P1598) for 2 hr at 37 °C.

For more info on this Assay Kit Cat# K2073, visit:

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BioVision, Inc. is a privately held Life Science company headquartered in the beautiful San Francisco Bay area. BioVision develops and offers a wide variety of products including assay kits, antibodies, recombinant proteins & enzymes, and other innovative research tools for studying Apoptosis, Metabolism, Cell Proliferation, Cellular Stress, Cell Damage and Repair, Diabetes, Obesity and Metabolic Syndrome, Stem Cell Biology, Gene Regulation, Signal Transduction, and more.

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