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Glycolysis Assays

March 2018

Glycolysis


The Glycolysis is practically found in almost all cells, prokaryotic and eukaryotic cells. This pathway gets highly-upregulated in tumor cells (Warburg effect). The enzymes involved in glycolysis are potential targets for the cancer treatment. BioVision offers the most complete series of assays measuring various metabolites, cofactors, and enzymes involved in the glycolytic pathway.

Key Features:

• Non-radioactive, homogeneous assays
• Specific assays
• Convenient: minimal sample preparation; fast protocols (1-2 hours )
• Cost effective:100 assays; High Throughput Screening compatible
• Validated: using mammalian tissues, cells, biological fluids


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Description

ADP/ATP Ratio Bioluminescence Assay Kit, ApoSENSOR

K255: ADP/ATP Ratio Bioluminescence Assay Kit, ApoSENSOR

The ApoSENSOR™ ADP/ATP Ratio Assay kit utilizes bioluminescent detection of the ADP and ATP levels for a rapid screening of apoptosis, necrosis, growth arrest, and cell proliferation simultaneously in mammalian cells.

NAD/NADH Quantitation Colorimetric Kit

K337: NAD/NADH Quantitation Colorimetric Kit

BioVision’s NADH/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio.

PicoProbe™ NADH Fluorometric Assay Kit

K338: PicoProbe™ NADH Fluorometric Assay Kit

BioVision’s PicoProbe™ NADH Assay kit provides a highly sensitive method to detect low level of NADH.

NADP/NADPH Quantitation Colorimetric Kit

K347: NADP/NADPH Quantitation Colorimetric Kit

The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio.

ATP Colorimetric/Fluorometric Assay Kit

K354: ATP Colorimetric/Fluorometric Assay Kit

BioVision newly developed ATP Colorimetric and Fluorometric Assay kit is designed to be a robust, simple method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric or fluorometric methods.

ADP Colorimetric/Fluorometric Assay Kit

K355: ADP Colorimetric/Fluorometric Assay Kit

BioVision ADP Assay kit II is suitable for measuring ADP levels in samples that contain reducing substances, which may interfere with oxidase-based assays.

ADP Colorimetric Assay Kit II

K356: ADP Colorimetric Assay Kit II

BioVision’s ADP Assay kit II is suitable for measuring ADP levels in samples that contain reducing substances, which may interfere with oxidase-based assays.

PEP Colorimetric/Fluorometric Assay Kit

K365: PEP Colorimetric/Fluorometric Assay Kit

BioVision’s phosphoenolpyruvate (PEP) Assay Kit provides a convenient colorimetric and fluorometric means to measure PEP levels in various samples.

Glucose Colorimetric/Fluorometric Assay Kit

K606: Glucose Colorimetric/Fluorometric Assay Kit

Glucose level is a key diagnostic parameter for many metabolic disorders. Measurement of glucose can be very important in both research and drug discovery processes. This Assay Kit provides direct measurement of glucose in various biological samples.

Pyruvate Colorimetric/Fluorometric Assay Kit

K609: Pyruvate Colorimetric/Fluorometric Assay Kit

BioVision provides a simple, direct and automation-ready procedure for measuring pyruvate concentration in various biological samples.

Glucose-6-Phosphate Colorimetric Assay Kit

K657: Glucose-6-Phosphate Colorimetric Assay Kit

BioVision's glucose-6-phosphate Assay Kit is a simple, sensitive and rapid means of quantifying G6P in a variety of samples.

Aldolase Activity Colorimetric Assay Kit

K665: Aldolase Activity Colorimetric Assay Kit

Aldolase Activity Assay, aldolase converts fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone, reduces a nearly colorless probe to a colored product with absorbance at 450 nm.

Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kit

K670: Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kit

BioVision’s Triose Phosphate Isomerase Activity Assay kit provides a quick and easy way for monitoring Triose Phosphate Isomerase activity in a variety of samples.

PicoProbe™ Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit

K673: PicoProbe™ Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit

Biovision’s PicoProbe™ Dihydroxyacetone Phosphate Assay kit is suitable for measuring low levels of DHAP typically found in a variety of samples.

GAPDH Activity Assay Kit

K680: GAPDH Activity Assay Kit

BioVision’s GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase; EC 1.2.1.12) GAPDH Activity Assay Kit provides a simple and sensitive method for monitoring GAPDH activity in various samples.

Glucose Colorimetric Assay Kit II

K686: Glucose Colorimetric Assay Kit II

BioVision’s Glucose Assay Kit II provides direct measurement of glucose in various biological samples.

PicoProbe™ Glucose-6-Phosphate Fluorometric Assay Kit

K687: PicoProbe™ Glucose-6-Phosphate Fluorometric Assay Kit

BioVision's glucose-6-phosphate Assay Kit is a simple, sensitive and rapid means of quantifying G6P in a variety of samples.

PicoProbe™ Glucose Fluorometric Assay Kit

K688: PicoProbe™ Glucose Fluorometric Assay Kit

Biovision’s PicoProbe™ Glucose Assay kit is simple, rapid, ultra-sensitive and suitable for high-throughput.

Enolase Activity Colorimetric/Fluorometric Assay Kit

K691: Enolase Activity Colorimetric/Fluorometric Assay Kit

This simple & sensitive assay Kit can detect enolase activity less than 0.04 mU in a variety of samples.

Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit

K709: Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit

BioVision provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc.

Human Hexokinase (HK) Inhibitor Screening Kit (Colorimetric)

K713: Human Hexokinase (HK) Inhibitor Screening Kit (Colorimetric)

BioVision’s Human Hexokinase II (HKII) Inhibitor Screening kit uses the HKII’s ability to convert glucose into glucose-6-phosphate.

PicoProbe™ Hexokinase Activity Assay Kit (Fluorometric)

K769: PicoProbe™ Hexokinase Activity Assay Kit (Fluorometric)

BioVision’s PicoProbe™ Hexokinase Activity Assay kit provides a quick and easy method for monitoring HK activity in a wide variety of samples.

Phosphoglucose Isomerase Activity Colorimetric Assay Kit

K775: Phosphoglucose Isomerase Activity Colorimetric Assay Kit

BioVision’s Phosphoglucose Isomerase Activity Assay Kit, the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase.

Phosphofructokinase (PFK) Activity Colorimetric Assay Kit

K776: Phosphofructokinase (PFK) Activity Colorimetric Assay Kit

BioVision’s Phosphofructokinase Activity Assay kit, PFK converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP.

2-Phosphoglycerate Colorimetric/Fluorometric Assay Kit

K778: 2-Phosphoglycerate Colorimetric/Fluorometric Assay Kit

BioVision’s 2-Phosphoglycerate Assay kit is a sensitive, fast and easy-to-use kit. In this assay, 2PG is converted by Enzyme Mix to PEP, which is further converted to pyruvate.

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