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Glutamate Dehydrogenase Inhibitor Screening Kit (Fluorometric)

June 2019

Glutamate Dehydrogenase Inhibitor Screening Kit (Fluorometric)

for high-throughput screening of GDH inhibitors


MILPITAS, Calif. - June 17, 2019 - PRLog -- Glutamate dehydrogenase (GDH) ( is a hexameric enzyme that catalyzes the reversible conversion of glutamate to α-ketoglutarate and ammonia while reducing NAD+ to NADH. Glutamate can be converted to α-ketoglutarate by GDH and then fluxed into the TCA cycle where it can be used to support oxidative phosphorylation, production of lipids, or to replenish key intermediates such as oxaloacetate. It is a key enzyme in the glutamine metabolic pathway. Overexpression of GDH has been shown to promote cell proliferation, migration and invasion in several tumors such as colorectal cancer. The increased reliance on glutamine metabolism makes GDH a target for therapeutic intervention in cancer. GDH inhibitors have been shown to be toxic for cancer cells in vitro. In BioVision's Glutamate Dehydrogenase inhibitor screening Kit, Glutamate is deaminated by GDH producing NADH, which converts a non-fluorescent probe to a fluorescent product in the presence of enzyme mix that is detected fluorometrically at Ex/Em = 535/587 nm. The kit provides a rapid, simple, sensitive plate based test, which is also suitable for high-throughput screening of GDH inhibitors.

Figure:  Enzyme kinetics in presence and absence of GDH Inhibitor. Assays were performed using kit K185-100 protocol.

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About BioVision:
BioVision, Inc. is a privately held Life Science company headquartered in the beautiful San Francisco Bay area. BioVision develops and offers a wide variety of products including assay kits, antibodies, recombinant proteins & enzymes, and other innovative research tools for studying Apoptosis, Metabolism, Cell Proliferation, Cellular Stress, Cell Damage and Repair, Diabetes, Obesity and Metabolic Syndrome, Stem Cell Biology, Gene Regulation, Signal Transduction, and more.

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