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EZClick™ Myristoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

May 2019

EZClick™ Myristoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

Labeling and detection of myristoylated proteins K497-100 from BioVision


MILPITAS, Calif. - May 16, 2019 - PRLog -- Myristoylation is a co- and post-translational modification of proteins. Myristoylated proteins are involved in cellular trafficking, some cellular functions and oncogenesis. In this modification, Myristic acid, a saturated fatty acid is covalently bond to a protein through the action of N-myristoyl transferase (NMT). NMT transfers the myristate moiety from Myristoyl-CoA to a N-terminal glycine of a target protein. NMT is associated with carcinogenesis, the upregulation of its expression and activity is observed in colorectal cancer, gall-bladder cancer
and brain tumors.
BioVision offers EZClickTM Myristoylated Protein Assay Kit, a highly specific, simple and robust method for labeling and detection of myristoylated proteins. We use Alkynyl Myristic Acid that is fed directly into the cells and gets  incorporated into proteins during or post translation, followed by click reaction with and azide-containing dye. This system offers a powerful method for imaging localization, trafficking, and dynamics of myristoylated proteins, or detection by FACS for quantitative studies. Labeled myristoylated proteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-azide or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format.

Figure: Jurkat cells: FACS analysis of negative control (Black), background (EZClick only, Green), positive control (Myristic Acid Label and EZClick, Pink). Signal measured in FL-1 channel clearly shows the Myristic Acid Labeling of Protein. Assays were performed using K497 kit protocol.

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About BioVision:
BioVision, Inc. is a privately held Life Science company headquartered in the beautiful San Francisco Bay area. BioVision develops and offers a wide variety of products including assay kits, antibodies, recombinant proteins & enzymes, and other innovative research tools for studying Apoptosis, Metabolism, Cell Proliferation, Cellular Stress, Cell Damage and Repair, Diabetes, Obesity and Metabolic Syndrome, Stem Cell Biology, Gene Regulation, Signal Transduction, and more.

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