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EZClick™ Global Protein Synthesis Assay Kit (K467-100)

August 2018

EZClick™ Global Protein Synthesis Assay Kit (K467-100)


MILPITAS, Calif. - Aug. 9, 2018 - PRLog -- Protein synthesis is a tightly regulated process and many critical controls in gene expression occur at the level of translation to ensure that production of specific cellular proteins is quickly turned on/off under specific conditions (heat shock, starvation, etc). It is essential in cell growth, proliferation, signaling, differentiation or death; therefore, the identity and amount of the synthesized proteins are critical in determining the physiological state of the cell. Methods of detection and characterization of nascent proteins, or changes in spatial and temporal protein expression/degradation patterns during disease, drug treatments or environmental changes are important for assessment of cytotoxicity. Biovision's EZClick™ Global Protein Synthesis Assay Kit (K467-100) utilizes a novel chemical method based on alkyne analog of puromycin, O-Propargyl-puromycin (OP-puro). OP-puro stops translation by forming covalent conjugates with nascent polypeptide chains. Truncated polypeptides are rapidly turned over by the proteasome and can be detected based on a click reaction with the fluorescent azide (Ex/Em= 494/521 nm). OP-puro does not require methionine-free conditions and can be used to label nascent proteins directly in the cell culture. We provide sufficient materials for 100 simple and specific assays to detect nascent proteins synthesized under various physiological conditions in the real-time, and Cycloheximide, an inhibitor of protein synthesis that serves as a control.

Figure: Inhibitory effect of Cycloheximide on nascent polypeptides synthesis using K467-100. Jurkat cells (1X106 cells/well) or were pre-treated with vehicle or Cycloheximide for 30 min at 37°C followed by incubation with EZClick™ Protein Label or EZClick™ Protein Label and Cycloheximide for additional 3 h then processed for detection according to the kit protocol.

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About BioVision:
BioVision, Inc. is a privately held Life Science company headquartered in the beautiful San Francisco Bay area. BioVision develops and offers a wide variety of products including assay kits, antibodies, recombinant proteins & enzymes, and other innovative research tools for studying Apoptosis, Metabolism, Cell Proliferation, Cellular Stress, Cell Damage and Repair, Diabetes, Obesity and Metabolic Syndrome, Stem Cell Biology, Gene Regulation, Signal Transduction, and more.

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