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EZCell™ Calcium Detection Kit (Cell-Based) (K432-50)

May 2018

EZCell™ Calcium Detection Kit (Cell-Based) (K432-50)


MILPITAS, Calif. - May 9, 2018 - PRLog -- Calcium is essential for all living organisms. Calcium sequestration and release into the cytoplasm function as a signal for many cellular processes. As an important second messenger, calcium is involved in many physiological processes such as nerve impulse transmission, muscle contraction, etc. Intracellular calcium is mainly stored in mitochondria and endoplasmic reticulum, and its concentration in cytosol is tightly regulated. Dysfunction of calcium homeostasis causes oxidative stress, cell death and pathogenesis of Alzheimer's disease. BioVision has developed an EZCell™ Calcium Staining Kit that contains membrane permeable, intracellular calcium probe. Upon cell entry, the calcium probe is hydrolyzed by intracellular esterases rendering it membrane impermeable. The cleaved calcium probe binds to intracellular calcium generating fluorescence. The intensity of fluorescence is directly proportional to intracellular calcium levels. A Positive Control reagent is also provided, and serves as experimental control, which elevates intracellular Calcium levels resulting in increased calcium staining. This easy-to-use, non-radioactive kit allows studying the regulation of calcium at the cellular level by using Fluorescence Microscopy and Flow Cytometry in cultured cells.

Figure: Calcium Staining in Jurkat and HeLa cells using K432-50. 1x106 Jurkat or 1x105 HeLa cells were treated with vehicle or Positive Control reagent for 30 minutes. Followed by washes, cells were incubated in 250 µl of medium + 0.5% FBS with 1X Intracellular Calcium Staining for 45 minutes at 37°C, according to kit's protocol. Panel A: Fluorescence microscope images of Positive Control Staining (basal calcium staining; left panel) and Cells treated with Experimental Control reagent (middle panel). Increased fluorescence induced by Positive Control reagent compared to the Basal Calcium levels confirms intracellular accumulation of Calcium in the cells. Right panel high resolution images of cells treated with Positive Control reagent.

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About BioVision:
BioVision, Inc. is a privately held Life Science company headquartered in the beautiful San Francisco Bay area. BioVision develops and offers a wide variety of products including assay kits, antibodies, recombinant proteins & enzymes, and other innovative research tools for studying Apoptosis, Metabolism, Cell Proliferation, Cellular Stress, Cell Damage and Repair, Diabetes, Obesity and Metabolic Syndrome, Stem Cell Biology, Gene Regulation, Signal Transduction, and more.

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