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Alpha Galactosidase Activity Assay Kit (Fluorometric) (K407-100)

May 2018

Alpha Galactosidase Activity Assay Kit (Fluorometric) (K407-100)


MILPITAS, Calif. - May 15, 2018 - PRLog -- Alpha-Galactosidase -α-Gal; EC hydrolyzes alpha-galactosyl moieties found in glycolipids and glycoproteins. In mammals, α-Gal hydrolyzes poly- and oligosaccharides commonly found in dietary sources that are difficult to digest. Therefore, α-Gal is used in dietary supplements that help to reduce the production of intestinal gases due to consumption of certain foods. It is known total α-Gal activity is due to two major isozymes with unique, yet different thermostability profiles. Alpha-Galactosidase A, is thermolabile and represents approximately 90% of total α-Gal activity. Fabry Disease, a lysosomal disease disorder, is characterized by mutations in alpha-Galactosidase A. These mutations cause abnormal accumulation of glycosphingolipids in lysosomes. BioVision's Alpha Galactosidase Activity Assay Kit provides a simple, rapid way to monitor total α-Gal activity in wide variety of biological samples. In this kit, α-Gal cleaves a synthetic specific substrate releasing a fluorophore, which can be easily quantified (Ex/Em= 360/445 nm). The assay is specific, sensitive and can detect as low as 0.1 µU of α-Galactosidase activity.

Figure: α-Galactosidase Activity in Mouse Kidney Tissue Extracts (1 µg protein) and U937 Cell Lysates (0.2 µg protein) using K407-100.

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BioVision, Inc. is a privately held Life Science company headquartered in the beautiful San Francisco Bay area. BioVision develops and offers a wide variety of products including assay kits, antibodies, recombinant proteins & enzymes, and other innovative research tools for studying Apoptosis, Metabolism, Cell Proliferation, Cellular Stress, Cell Damage and Repair, Diabetes, Obesity and Metabolic Syndrome, Stem Cell Biology, Gene Regulation, Signal Transduction, and more.

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