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Aldehyde Dehydrogenase 2 Inhibitor Screening Kit (Fluorometric)

August 2020

High Sensitivity Carbohydrate Assay Kit (Colorimetric)

A simple, sensitive, robust assay for the quantification of carbohydrates even when present in low concentration.


MILPITAS, Calif. - Aug. 17, 2020 - PRLog -- Aldehyde Dehydrogenase 2 (ALDH2), mitochondrial is a member of the ALDH superfamily that participates in multiple metabolic pathways including the oxidation of toxic biogenic and environmental aldehydes. ALDH2 is a key enzyme located in the mitochondrial matrix. It plays an important role in ethanol metabolism by converting acetaldehyde, a carcinogenic metabolite of ethanol to acetate. Imbalances of ALDH2 have been linked to both alcoholism and alcohol sensitivity in people. Reduced ALDH2 activity has been strongly correlated with reduced alcohol consumption and reduced incidences of alcoholism, which led to the development of selective ALDH2 inhibitors as antidipsotropic or alcohol aversive agents for treating alcoholism. Disulfiram, an alcohol-aversive drug inhibiting ALDH2 has been approved by the FDA and is used clinically for over 40 years for treating alcohol dependency. However, the drug is a non-specific alkylating agent leading to many adverse side effects and drug-drug interactions. Thus, novel and specific inhibitors of ALDH2 free of the negative side effects could be used in aversion therapy.

BioVision's Aldehyde Dehydrogenase 2 Inhibitor Screening Kit provides a quick and sensitive way of screening, studying and characterizing potential inhibitors of ALDH2. In this assay, acetaldehyde is oxidized by ALDH2 to form NADH, which then couples with PicoProbeTM to generate a fluorescence signal measured at Ex/Em = 535/587 nm. The percentage inhibition can be determined by comparing the activity of ALDH2 treated with test inhibitor(s) vs untreated control.

Figures: (a). D-Glucose Standard Curve. (b). Absorbance of Monosaccharides upon reacting with High Sensitivity (HS) Carbohydrate Developer and Phenol. (c). Total Polysaccharide content in Pneumococcal Polysaccharide Powder Type 4 (US Type 4), calculated total carbohydrate concentration (glucose equivalent) is 0.47 ± 0.02 μg/μl. The assay was performed according to the kit assay protocol.

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About BioVision:

BioVision, Inc. is a privately held Life Science company headquartered in the beautiful San Francisco Bay area. BioVision develops and offers a wide variety of products including assay kits, antibodies, recombinant proteins & enzymes, and other innovative research tools for studying Apoptosis, Metabolism, Cell Proliferation, Cellular Stress, Cell Damage and Repair, Diabetes, Obesity and Metabolic Syndrome, Stem Cell Biology, Gene Regulation, Signal Transduction, and more.

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