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3D Culture HTS Cell Viability Assay Kit (Colorimetric)

January 2020

3D Culture HTS Cell Viability Assay Kit (Colorimetric)

Non-radioactive and high-throughput method for characterizing and screening cell viability and cytotoxicity


MILPITAS, Calif. - Jan. 20, 2020 - PRLog -- Three-dimensional (3D) cell cultures are artificially-created environments, where cells are allowed to grow or interact with their surroundings in a 3D fashion. 3D cell cultures improve the function, differentiation and viability of cells and recapitulate the in vivo microenvironment as compared to conventional 2D cell cultures. 3D matrices provide a physiologically relevant screening platform by mimicking the in vivo responses for many cell types including cancer and stem cells. This is specifically important in developmental morphogenesis, pharmacology, drug metabolism and drug toxicity studies. Additionally, quantification of the number of viable cells is an indispensable tool in in vitro screening in these studies. However, with the use of 3D matrices, some protease-based dissociation methods don't completely dissolve the matrices or the cell aggregates, which may alter the result of the in vitro viability assessment quantitatively. BioVision's 3D Culture Cell Viability Assay Kit provides a standardized colorimetric method for sensitive quantification of viable cells that can detect as high as 250,000 viable cells and as low as 1000 viable cells in each well. The absorbance is measured at 460 nm. The measured intensity is proportional to the number of viable cells. Further, the kit comes with an optimized and gentle non-enzymatic dissociation solution for the recovery of viable and dead cells from spheroids in matrices and scaffolds. This assay kit provides an easy-touse, non-radioactive, high-throughput method for characterizing and screening cell viability and cytotoxicity.

Figure: (a) Standard Curve for viable HeLa cells (0 to 250,000 cells). (b) Standard Curve for viable HepG2 cells (0 to 100,000 cells). (c). Viable cells (HepG2) from 3D culture following 48 hour treatment with Cisplatin. Cells were cultured in 3D Cell Culture BME Matrix (Biovision Cat. #K518) for 18 days to form spheroids. Spheroids were treated as described above and the viable cells determined according to the kit K2032 protocol.

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About BioVision:

BioVision, Inc. is a privately held Life Science company headquartered in the beautiful San Francisco Bay area. BioVision develops and offers a wide variety of products including assay kits, antibodies, recombinant proteins & enzymes, and other innovative research tools for studying Apoptosis, Metabolism, Cell Proliferation, Cellular Stress, Cell Damage and Repair, Diabetes, Obesity and Metabolic Syndrome, Stem Cell Biology, Gene Regulation, Signal Transduction, and more.

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